摘要
目的:建立肉瘤S180鸡胚尿囊膜(CAM)移植瘤模型,研究其形态学及生物学特性。方法:在无菌条件下将肉瘤S180肿瘤细胞接种于CAM上,观察影响肉瘤S180移植瘤成活的因素、移植瘤生长特性、生物学性状和形态学特征。结果:建立了肉瘤S180 CAM移植瘤模型。移植瘤易于生长,成瘤率较高,具有较强的血管生成诱导作用。当接种肉瘤S180肿瘤细胞为5×107个/枚时,成瘤率达到83.33%,且与第1天比较,诱导血管数从第2天开始就呈统计学差异P<0.05,当移植第4天时就呈极显著性差异P<0.001。结论:该模型具有易于复制、操作简单、适用范围广等特点,可应用于肿瘤血管生成的生物学行为研究,为抗血管生成抗肿瘤药物的筛选提供了更为直观的平台。
Objective：To establish S180 sarcoma model on the chick embryo chorioallantoic membrane（CAM） and to study its morphological and biological properties.Method： S180 cell was cultivated and implanted on the CAMs under sterile conditions.The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied.Result： S180 sarcoma model on the CAM was established.The implanted tumor was easy to grow on the CAM,and had a strong tendency of angiogenesis.When the number of inoculated tumor cells reached 5×107 in each egg,the tumor rate could reach 83.33%.Compared with the first day,the number of induced vessels on the second day was significantly different（P0.05）.On the fourth day after transplant it was greatly increased（P0.001）.Conclusion： Our findings suggest that this model is of easy duplication and simple operation,and can be widely used in the research of the biological properties for tumor angiogenesis and it provides an intuitive platform for the drug screening of anti-angiogenic.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期221-225,共5页
Chinese Journal of Experimental Traditional Medical Formulae
作者简介
袁野，硕士研究生，从事中药药理新药研究与开发研究，Tel：15195555725，E-mail：yuanye2008@126．com
[通讯作者]李吉萍，硕士，香港大学访问学者，副教授，硕士研究生导师，从事中药药理新药研究与开发研究，Tel：13815833998．E-mail：jipingli2005@126．com
