摘要
目的建立胆管癌细胞系裸鼠肝门部胆管原位种植瘤模型。方法应用自制显微注射针将培养的胆管癌细胞系QBC939细胞接种于裸鼠肝门部胆管与门静脉组织间隙紧贴胆管处,15d后行种植瘤组织解剖学和病理学检查。结果每只裸鼠肝门部胆管与门静脉组织间隙内可接种浓度为1×107个/ml的胆管癌细胞悬液100μl;原位种植15d后,肝门部胆管原位种植瘤成瘤率为100%(7/7);病理学观察显示为典型的瘤组织表现。结论成功建立了胆管癌细胞系裸鼠肝门部胆管原位种植瘤模型。
Objective To establish the implantation model of human in situ hilar cholangiocarcinoma cell line in nude mice.Methods Cultured human hilar cholangiocarcinoma cell line QBC939 was injected into the space between the hilar bile duct and portal vein of nude mice with a micro-injector.Anatomical and pathological features of the mice were detected 15d after the implantation model of human in situ hilar cholangiocarcinoma cell line was established.Results Suspension of 100ml of hilar cholangiocarcinoma cells at the concentration of 1×106/ml was injected into the space between the hilar bile duct and portal vein of each nude mouse.The successful rate was 100% 15d after the implantation model of human in situ hilar cholangiocarcinoma cell line.Histological examination showed typical tumor tissues in mice.Conclusion An implantation model of human in situ hilar cholangiocarcinoma can be established in nude mice.
出处
《军医进修学院学报》
CAS
2011年第5期511-512,518,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
胆管肿瘤
模型
动物
小鼠
裸
Bile Duct Neoplasms
Models
Animal
Mice
Nude
作者简介
李成刚，博士，主治医师。专业方向为肝门部胆管癌的基础与临床研究。Email：lcgang301@126．com
