摘要
目的:探讨棕色脂肪组织(brown adipose tissue,BAT)、解偶联蛋白1(uncoupling protein 1,UCP1)、高脂饮食和中药干预在实验动物能量代谢中的关联性和机制。方法:动物随机分为4组。每组16只。①对照组:普通饲料喂养,d70～d98每天按10 g.kg-1体重生理盐水ig。d98检测刺激性产热,观察2 h肛温变化曲线和温度峰值,计算温度曲线下面积;②阳虚组:d42手术切除肩胛间BAT,余同①组;③脾阳虚组:d49～d98高脂饲料(83%普通饲料,15%甘油三酯,2%胆固醇)喂养;隔日19℃环境喂养,余同②组;④中药组:d70～d98每天按4 g.kg-1体重附子理中汤ig。余同③组。每周测体重1次。以效能体重增长率、效能产热温度峰值、产热温度曲线下面积为参考指标。取BAT测UCP1表达量。结果:①效能体重增长率,脾阳虚组与阳虚组相比体重增长减慢(P<0.01);中药组与脾阳虚组相比,体重增长加快(P<0.01)。②效能产热温度峰值,脾阳虚组与阳虚组相比效能产热温度峰值升高(P<0.01);中药组与脾阳虚组相比,效能产热温度峰值升高(P<0.01);中药组与脾阳虚组相比,效能体重增长率与效能产热温度峰值变化程度呈正相关。③产热温度曲线下面积,脾阳虚组与阳虚组相比产热曲线下面积增加(P<0.05);中药组与脾阳虚组相比,产热温度曲线下面积增加(P<0.01)。效能体重增长率与产热温度曲线下面积变化程度呈正相关。中药组上调UCP1表达量,和阳虚组以及脾阳虚组相比,差异均具有高度显著性(P<0.01)。结论:温阳健脾中药促使高脂饮食转化为BAT并提高其产热活性。温阳健脾中药上调UCP1在BAT中的表达,通过UCP1的解偶联作用,使BAT分解产热而改善阳虚畏寒的症状。
Objective：To explore the relationship and mechanism about brown adipose tissue,uncoupling protein 1（UCP1）,high fatty diet and Chinese herbal decoction during the process of energy metabolism.Method： Rats were divided into four groups（n=16 each）.①Control group was fed with ordinary food,also ig given 0.9% NaCl 10 g·kg-1 during d70-d98 daily.The ability of ISO deducing heat generating was tested on d98,and the curve of the rectal temperature was monitored,and the peak within two hours and the area under the curve were determined.②Yang deficiency syndrome group： the same procedure was carried out as the control group except removing brown adipose tissue on d42.③Spleen yang deficiency syndrome group： the same procedure was carried out as the yang deficiency syndrome group except of being fed with high fatty diet（including 83% ordinary diet,15% triglycerides,2% cholesterol） from d49 to d98 and placed in 19 ℃ environment every other day.④Chinese herbal decoction group： the same procedure was carried out as the spleen yang deficiency syndrome group except giving 4 g·kg-1 ig daily with Aconitum Lizhong decoction and the body weight was measured every week.The index of the weight growth rate,the peak of temperature and the area under the temperature curve were all investigated.The content of UCP1 was determined by using brown adipose tissue.Result： ①Weight growth rate： compared with yang deficiency syndrome group,weight growth rate in spleen yang deficiency syndrome group was lower（P0.01）;compared with spleen yang deficiency syndrome group,weight growth rate was higher in the Chinese herbal decoction group（P0.01）.②Temperature： compared with yang deficiency syndrome group,the temperature peak of heat generating in spleen yang deficiency syndrome group was higher（P0.01）;compared with spleen yang deficiency syndrome group,temperature peak of heat generating was higher in Chinese herbal decoction group（P0.01）.There was a positive correlation between weight growth rate and temperature peak of heat generating.③Area under the temperature curve： Compared with yang deficiency syndrome group,the area in spleen yang deficiency syndrome group was increased（P0.01）;compared with spleen yang deficiency syndrome group,the area was increased in Chinese herbal decoction group（P0.01）.There appeared a positive correlation between weight growth rate and area under the temperature curve.Compared with yang deficiency syndrome group and spleen yang deficiency syndrome group,Chinese herbal decoction group up-regulated content of UCP1,and the difference was highly significant（P0.01）.Conclusion：Chinese herbal decoction with function of heat generating and tonifying spleen is inclined to make high fatty diet turn into brown adipose tissue and advance its activity.Chinese herbal decoction with function of warming middle energizer and tonifying spleen can upgrade level and expression of UCP1 in brown adipose tissue,and then make brown adipose tissue decomposed by the mechanism of uncoupling with UCP1,finally dispelling coldness from the bodies in the rats with yang deficiency syndrome.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期206-210,共5页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
脾阳虚证
棕色脂肪组织
解偶联蛋白1
关联性
spleen yang deficiency syndrome
brown adipose tissue
uncoupling protein 1
correlation
作者简介
吴云起，本科，主治医师，从事中西医结合骨科学的研究，Tel：0779-2038081
[通讯作者]唐汉庆，博士，讲师，从事中西医结合基础研究，Tel：0776-2420488
