摘要
目的:以血府逐瘀汤加不同剂量黄芪为基本方,通过检测和观察缺氧缺血性脑损伤SD大鼠脑组织内神经生长因子(NGF mRNA)的表达、神经行为学改变和脑组织病理改变来探讨中医益气活血法的脑保护作用并阐述其机制。方法:断奶2周SD大鼠80只,体重约100g,雌雄各半。随机分成5组(n=16),对照组(又称假手术组);模型组、中药1、2、3组(统称手术组),除对照组外,均结扎双侧颈总动脉,建立脑缺氧缺血模型,术后分别以生理盐水(对照组、模型组)、血府逐瘀汤(为中药1组)、每剂血府逐瘀汤加10g黄芪(为中药2组)和每剂血府逐瘀汤加40g黄芪(为中药3组)1mL/(100g.d)灌胃。术后1天和6天观察神经学行为,进行Zea Longa神经功能评分,断头取脑,光镜下观察脑组织HE染色的病理改变,荧光定量RT-PCR检测脑组织内NGF mRNA含量。结果:术后第1天,各组与对照组比较出现明显的神经功能障碍(P<0.05)和病理改变,脑组织内NGF mR-NA含量也明显增加(P<0.05)。术后第6天,中药各组与模型组比较神经功能障碍和病理表现均有明显改善(P<0.05),脑组织内NGF mRNA含量均有显著增加(P<0.05),中药各组间增加无显著差异。术后第6天与第1天比较,模型组神经功能、病理表现均无明显改善,NGF mRNA表达有明显减少(P<0.05),中药各组神经功能、病理表现均有明显改善(P<0.05),NGF mRNA表达增加无明显差异。结论:血府逐瘀汤加黄芪灌胃能明显改善大鼠缺氧缺血性脑损伤后的神经行为学和脑组织病理学改变,显著增加脑组织内NGF mRNA的表达,并以血府逐瘀汤加适量黄芪(10g)疗效较佳,是治疗缺氧缺血性脑损伤的有效方法。
Objective：To explore the brain protecting effect based on Xuefuzhuyu decoction plus different dosages of Radix Astragali by testing the amount of the nerve growth factor mRNA（NGF mRNA）,evaluating neurological behaviors,and observing the pathologic changes of brain tissue in SD rats with hypoxic-ischemic encephalopathy（HIE）.To reveal the mechanism of the therapy of replenishing Qi and activating blood circulation.Methods：80 healthy clean SD rats（off weaning for two weeks,weight about 100g,40 male and 40 female rats）were divided into 5 groups（n=16）.The control group（also called the feigned operation group）,the model group and three Chinese medicine treating groups（also called the operation groups collectively）.Bilateral carotis ligature is performed in all groups except control group to establish HIE model.Gastric lavage is performed after operation with normal saline（control and model groups）,xuefuzhuyu decoction（Chinese medicine group1）,xuefuzhuyu decoction plus 10g radix astragali（Chinese medicine group2） and xuefuzhuyu decoction plus 40g radix astragali（Chinese medicine group3） respectively.Neurological behaviors were observed on the 1st day and the 6th day after operation and evaluated by Zea Longa neural function grading criteria.Brain tissues were obtained after decollation and pathologic changes were examined under light lens after HE dyeing.The amounts of NGF mRNA were tested with quantitative fluorescence RT-PCR.Results：On the 1st day after the operation,compared with control group,all the other groups had remarkable neurological malfunction（P0.05）,pathologic changes and significant increase in the amount of NGF mRNA（P0.05）.On the 6th day,three Chinese medicine treating groups had remarkable improvement in neurological malfunction,pathologic changes（P0.05） and a significant increase in the amounts of NGF mRNA compared with the model group（P0.05）.There is no significant difference among three Chinese medicine groups.Compared with the 1st day,a significant decrease of the amounts of NGF mRNA（P0.05） was found in the model group while neurological malfunction and pathologic changes remains no obvious improvement.For three Chinese medicine treated groups,remarkable improvement has been found both in neurological behavior and pathologic changes（P0.05） while no significant difference in increasing NGF mRNA expression among the three groups.Conclusions：Gastric lavaging xuefuzhuyu decoction with radix astragali can significantly improve both neurological behavior and pathological changes and significantly increase the NGF mRNA expression in SD rats with HIE.Chinese medicine treating group2（gastric lavaging xuefuzhuyu decoction with 10g radix astragali） has the best effect of pathological recovery among all groups.The therapy of replenishing Qi and activating blood circulation is an effective way for protecting the brain impairment caused by hypoxic-ischemic encephalopathy.
出处
《中华中医药学刊》
CAS
2011年第7期1552-1555,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药管理局资助项目(2004c118)
关键词
益气活血法
缺氧缺血性脑病
脑保护
therapy of replenishing Qi and activating blood circulation hypoxic-ischemic encephalopathy
brain protection
作者简介
吕中（1964-），男，浙江杭州人，主任医师，硕士，研究方向：中医及中西医结合心脑血管病基础与临床研究。
