摘要
用风味蛋白酶、复合蛋白酶和碱性蛋白酶对罗非鱼肉进行水解,以酶解液中氨基态氮含量为指标,选择出风味蛋白酶为水解罗非鱼蛋白的最适蛋白酶。单因素实验确定风味蛋白酶水解的最佳条件为:固液比为1:4、温度为50℃、未调节初始pH值(初始pH为6.9)、加酶量为0.6%(g/g,相对于鱼糜质量),酶解8 h后酶解液中氨基态氮含量为1.90 mg/g。
Flavuorzyme, protamex and alcalase were tested for the hydrolysis of tilapia by comparing the concentration of aminophenol nitrous in the hydrolyte. Flavourzyme was found as the best catalyst. The optimum hydrolytic conditions were determined as following: solid to liquid ratio 1:4, temperature 50℃, initial pH (6.9), enzyme dosage 0.6% (g/g), and hydrolysis time 8h, under which the value of aminophenol nitrous could reach 1.90 mg/g.
出处
《现代食品科技》
EI
CAS
2011年第6期678-680,共3页
Modern Food Science and Technology
作者简介
王凤祥(1987-),男,硕士研究生,研究方向:粮食、油脂及植物蛋白工程