摘要
目的探讨从中国皖南尖吻蝮蛇(agkistrodon acutus)蛇毒中提取的ACPC1,ACPC2对人肝癌BEL-7404细胞株的抑制作用。方法通过DEAE-sepharose fast flow阴离子交换层析、SP-sepharose fast flow阳离子交换层析、Sephadex G-75凝胶过滤层析及Butyl-S Sepharose 6 FF疏水柱从皖南尖吻蝮蛇蛇毒(Wannan agkistrodon acutus venom)中分离纯化出两个抗凝蛋白组分ACPC1、ACPC2;用MTT法检测ACPC1、ACPC2对人肝癌细胞Bel-7404的抑制作用。结果人肝癌细胞BEL-7404经ACPC1或ACPC2处理72h后,当ACPC1浓度≥3·125μg·mL-1,ACPC2浓度≥1·563μg·mL-1时,显示出明显的抑瘤作用,且呈剂量依赖关系。结论 ACPC1、ACPC2对体外培养的人肝癌细胞BEL-7404均具有明显的抑制作用。
Aim To investigate the antitumor activity of ACPC1 and ACPC2 from Agkistrodon acutus on human hepatocellular carcinoma BEL-7404 cells.Methods The ACPC1 and ACPC2 were isolated and purified from the venom of Wannan agkistrodon acutus with the use of ion-exchange chromatography of DEAE-Sepharose FF and SP-Sepharose FF,molecular sieve filtration through Sephadex G75 and hydrophobic chromatography of Butyl-S Sepharose 6 FF.The tumor-inhibitory effect of ACPC1 and ACPC2 on the human hepatocarcinoma BEL-7404 cells were detected by MTT assay.Result BEL-7404 cells were treated with ACPC1 or ACPC2 for 72 hours.When the concentration of ACPC1 was 3.125μg · mL-1 or more and the concentration of ACPC2 was 1.563μg · mL-1 or more,there was obvious inhibition on proliferation of human hepatocarcinoma BEL-7404 cell line.Conclusion Both of ACPC1 and ACPC2 have obvious inhibition on the human hepatocellular carcinoma BEL-7404 cells in vitro.
出处
《临床药物治疗杂志》
2011年第3期20-24,共5页
Clinical Medication Journal
基金
安徽省教育厅自然科学基础项目(KJ2007A039)
安徽省科技厅重点科研项目(07021018)
