摘要
目的评价脂多糖(LPS)诱发大鼠慢性阻塞性肺疾病(COPD)模型的可行性。方法气管内滴入脂多糖或生理盐水,每周1次,共8周。测定大鼠的气道阻力(RL)和肺动态顺应性(Cdyn),计数大鼠支气管肺泡灌洗液(BALF)中白细胞总数及分类,肺组织病理切片行HE和AB-PAS染色,并测定肺组织粘蛋白(MUC5AC)的含量。结果模型组大鼠RL明显升高(87.5%),Cdyn显著下降(16.4%);BALF中白细胞总数及分类中的中性粒细胞、淋巴细胞和单核巨噬细胞数均明显高于对照组;光镜下可见病变呈慢性支气管炎及肺气肿样改变。结论反复气管内滴入LPS可用于制备大鼠COPD模型,其肺功能、BALF细胞学及病理学改变符合人类COPD表现,可以用于实验研究。
Objective To establish and evaluate the feasibility of a rat model of chronic obstructive pulmonary dis-ease（COPD） induced by intratracheal instillation of lipopolysaccharide（LPS）.Methods LPS or saline was instilled in-tratracheally in rats once a week for consecutive 8 weeks.The rats were anesthetized and taken for the measurement of lung resistance（RL） and dynamic compliance（Cdyn）.Total and differential white blood cell counts in bronchoalveolar lavage fluid（BALF） were carried out.The pathological changes of the lung tissues were observed using HE and AB-PAS stai-ning,and the mucin 5AC content was measured.Results Intratracheal instillation of LPS caused a significant increase in RL（87.5%） and decrease in Cdyn（16.4%）.Total and differential white blood cell counts in BALF such as neutrophils,lymphocytes and macrophages were higher in the LPS-treated rats.Chronic bronchitis and emphysematous changes were ob-served as well.Conclusion A typical rat model of COPD can be induced by repeated intratracheal instillation of LPS,show-ing many features of human COPD in the lung function,BALF cytologic and pathologic changes.
出处
《中国实验动物学报》
CAS
CSCD
2011年第2期129-133,191,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
浙江省中医药重点项目研究计划资助项目(2008ZA014)
关键词
慢性阻塞性肺疾病
脂多糖
大鼠
模型
Chronic obstructive pulmonary disease
Lipopolysaccharide
Rat
Model
作者简介
刘君波，女，在读硕士研究生。
[通讯作者]唐法娣，Email：fadit@zju．edu．cn，Tel：13656667396
