摘要
目的:研究糖皮质激素诱发的亮氨酸拉链蛋白(glucocorticoid induced leucine zipper,GILZ)在多发性骨髓瘤(MM)骨病发展中的作用。方法:收集MM患者抗凝血11例,利用密度梯度离心法获取外周血单个核细胞(PBMCs),并将PBMCs分为地塞米松(DEX)干预组及空白对照组,相同条件培养48h。采用逆转录-酶联聚合反应(RT-PCR)法检测各组PBMCs中GILZ mRNA的表达;ELISA检测核因子κB受体激活剂配体(RANKL)及骨保护素(OPG)的表达。结果:在10-7 mol/L DEX刺激下,DEX刺激组MM患者PBMCs的GILZmRNA表达水平高于空白对照组(P<0.05);DEX刺激组OPG的表达量高于对照组(P<0.05),而RANKL的表达量诱导组与空白对照组相比差异无统计学意义(P>0.05)。结论:糖皮质激素可有效上调GILZ基因表达;GILZ的高表达可以调节MM患者RANKL及OPG的平衡,具有改善溶骨性损害的作用。
Objective：To investigate the function of glucocorticoid induced leucine zipper（GILZ） in multiple myeloma.Method：Peripheral blood mononuclear cells（PBMCs） were collected from multiple myeloma patients by Ficoll and divided into two groups： dexmamethasone（DEX） treated group and control group.Forty eight hours after treatment,total RNA were extracted in both groups.Reverse transcription polymerase chain reaction（RT-PCR） was used to analyze the expression of GILZ mRNA in PBMCs from both groups.Enzyme linked immunosorbent assay（ELISA） was used to analyze the expression of RANKL and OPG protein.Result：Both OPG and GILZ were increased in DEX treated group compared with control group（P0.05）.There is no difference of the expression of RANKL between two groups（P0.05）.Conclusion：DEX is an effective stimulator of GILZ production.Overexpression of GILZ can regulate the balance between OPG and RANKL,suggesting that GILZ may have therapeutic value for bone diseases,such as multiple myeloma.
出处
《临床血液学杂志》
CAS
2011年第3期293-296,共4页
Journal of Clinical Hematology
基金
湖北省卫生厅青年人才基金资助项目(No:QJX2008-25)
作者简介
通信作者：高清平，E-mail：gao．qingping@163．com
