摘要
目的检测腹腔感染时SD大鼠肠道粪便中肠源性防御素-5(rat defensin-5,RD-5)的变化,探讨其对肠黏膜屏障功能监测的意义。方法 SD大鼠80只,随机分成实验组和对照组,每组40只。实验组采用腹腔注射脂多糖(LPS)制作腹腔感染模型,剂量0.01 g/kg,对照组仅注射同等剂量生理盐水。分别于造模后2、4、8、12和24 h取血、粪便和末端回肠组织,检测血清二胺氧化酶(DAO)水平及粪便中RD-5水平,光镜下观察各组末端回肠黏膜形态学改变并对其损伤予以分级,并行血DAO、粪便RD-5和末端回肠损伤病理评分的相关性分析。结果对照组不同时间点末端回肠黏膜结构完整,实验组末端回肠黏膜和黏膜下层间质水肿、血管充血,黏膜层可见中性粒细胞浸润,肠绒毛毛细血管充血,部分区域有黏膜上皮脱落,浆膜层可见炎性渗出。与对照组同时间点比较,实验组血DAO水平在2 h即有明显升高(P<0.05),随着感染加重,8、12和24 h逐渐升高(P<0.05),与末端回肠损伤病理评分呈正相关。粪便中RD-5水平在2 h升高(P<0.05),而在4 h后降低(P<0.05),与末端回肠损伤病理评分无明显相关性,8、12和24 h降低显著(P<0.05),与末端回肠损伤病理评分呈负相关。结论粪便中RD-5含量变化可作为反映肠黏膜屏障功能状况的一种特异性指标。
【Objective】To investigate the value of fecal defensin-5 in supervising intestinal mucosa barri-er function in rats with abdominal infection.【Methods】 A total of 80 SD rats were randomly divided into two groups（n =40 each）： experimental group（intraperitoneal lipopolysaccharide）,control group.The rats were injected intraperitoneally with LPS（0.01 g/kg） or sterile saline.The levels of DAO in plasma and fecal RD-5 were measured and at 5 consecutive time points,2,4,8,12 and 24 h after modeling.The morphologic changes were observed and graded with microscopy.Analyze the correlation between the levels of DAO in plasma and fecal RD-5,the histopathological score of small intestinal mucosa membrane.【Results】 No ab-normal changes were observed with optical microscope in the small intestinal mucosa membrane of rats in the control group.In the experimental group,microscopic examination revealed interstitial edema,vascular en-gorgement and neutrophil infiltration in the small intestine mucosa membrane and the submucosa.Plasma lev-els of DAO were increased in rats with abdominal infection（P 0.05）,positively correlated to the small in-testinal mucosa membrane histopathological score.In contrast,8,12,and 24 h,fecal RD-5 level were de-ceased in rats with abdominal infection（P 0.05）,negatively correlated to the small intestinal mucosa mem-brane histopathological score.【Conclusion】Fecal RD-5 level may be a sensitive marker to reflect the intesti-nal mucosa barrier function.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第11期1292-1296,共5页
China Journal of Modern Medicine
基金
全军"十一五"计划专项课题(No:06Z017)
关键词
腹腔感染
防御素-5
二胺氧化酶
脂多糖
肠黏膜屏障
abdominal infection
Defensin-5
diamine oxidase
lipopolysaccharide
intestinal mucosa barri-er
作者简介
【通信作者】嵇武，E—mail：jiwusky@yaahoo．com．cn；Tel：025—80860134
