摘要
目的检测实验性重症急性胰腺炎时,SD大鼠小肠防御素-5 mRNA表达的变化,从分子水平上探讨胰腺炎时肠黏膜屏障受损的机制。方法40只SD大鼠随机分成4组,每组10只。模型组(B～E组)改良Aho’s法造模,于造模后第12,24和36小时处死,正常对照组(A组)直接处死。取血送淀粉酶检查,胰腺送病理检查。用Quanty One软件分析PCR产物凝胶图像以检测各组防御素-5 mRNA的表达变化。结果改良Aho’s法导致了SD大鼠重症急性胰腺炎,12 h防御素-5 mRNA表达轻微下调,36 h下调最明显。结论防御素-5的获得性缺陷可能是胰腺炎时发生肠道细菌移位的重要原因之一。
Objecive To detect the changes of intestinal defensin-5 mRNA expression in SD rats with experi-mental severe acute pancreatitis and explore the mechanisms of bacterial translocation at the molecular level. Methods Forty SD rats were randomly divided into 4 groups with 10 in each group. Experimental groups （from B to D）, pancreatitis induced by modified Aho＇s method, were killed at 12, 24 and 36 hours after the injection. The control group （A） was killed directly. Amylase and pancreatic pathological alterations were observed. The amplification products of RT-PCR were electrophoresed and the images were analyzed by Quanty One software. Results Severe acute pancreatitis in SD rats were induced by modified Aho＇s method. Intestinal defensin-5 mRNA expression was down regulated slightly at 12th hour and most markedly at 36th hours. Conclusion Acquired defensin-5 deficiency may play an important role in the pathogenesis of bacterial translocation in severe acute pancreatitis.
出处
《肝胆胰外科杂志》
CAS
2007年第6期360-362,366,共4页
Journal of Hepatopancreatobiliary Surgery
基金
杭州市科技局基金项目(2004453Q18)
浙江省中医药管理局基金项目(2004C134)
关键词
胰腺炎
防御素-5
MRNA表达
severe acute pancreatitis
defensin-5
mRNA expression
作者简介
李钢（1957-），男，浙江杭州人，主任医师，教授。
