摘要
目的:本研究利用新型免疫荧光标记试剂-量子点(QDs)结合组织芯片技术检测细胞外基质金属蛋白酶诱导因子(EMMPRIN/CD147)、基质金属蛋白酶2(MMP-2)和P53蛋白在人肺癌组织中的表达,并探讨EMMPRIN与肺癌恶性生物学行为的关系。方法:利用QDs免疫荧光组织化学(QDs-IHC)技术分别检测人肺癌组织芯片中EMMPRIN、MMP-2和P53蛋白的表达,并利用QDs免疫荧光双标法同时检测了EMMPRIN与P53蛋白的共表达。结果:EMMPRIN、MMP-2和P53蛋白表达在肺癌组织的阳性率分别为:70.00%、77.14%和72.86%,与非癌变肺组织相比,差异均显著(P<0.05);而且与肺癌的TNM分期、淋巴结转移均显著相关(P<0.05),但与其它的临床病理特征均明显无关(P>0.05)。EMMPRIN和MMP-2、P53蛋白表达之间均呈显著正相关(P<0.01)。结论:EMMPRIN、MMP-2和P53蛋白均与肺癌的发生有关,EMMPRIN可能与MMP-2、P53协同促进肺癌的恶性进展。
AIM： To investigate the expression of extracellular matrix metalloproteinase inducer（EMMPRIN/CD147）,matrix metalloproteinase2（MMP-2） and P53 proteins in human lung cancer tissues and to explore the relationship between EMMPRIN protein and malignant biological behaviour of lung cancer. METHODS： Fluorescent semiconductor nanocrystals [also known as quantum dots（QDs）] were applied,which were nanometer-sized light-emitting particles and were emerging as a new class of fluorescent probes for cancer detection due to the unique optical and electronic properties.The technique of QDs immunofluorescence histochemistry（QDs-IHC） was used to detect the protein expression of EMMPRIN,P53 and MMP-2 in the human lung cancer microarray,and co-expression of EMMPRIN/P53 proteins was also simultaneously detected by double-labeling immunofluorescence. RESULTS： Compared with non-cancerous lung tissues,the positive rates of EMMPRIN,P53 and MMP-2 proteins in the lung cancer tissues were 70.00%,77.14% and 72.86%,respectively,and the differences were all significant（P〈0.05）.The positive rates of EMMPRIN,P53 and MMP-2 proteins were all significantly related with tumor staging（TNM stage） and lymph node metastasis（P〈0.05）.The positive correlation between EMMPRIN expression and protein levels of MMP-2 and P53 was observed（P〈0.01）. CONCLUSION： The protein expression of EMMPRIN,P53 and MMP-2 is correlated with the development of lung cancer.Malignant progression of lung cancer promoted by EMMPRIN may be closely related with the expression of MMP-2 and P53.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第3期488-493,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30900652)
浙江省中医药普通课题研究计划资助项目(No.2008CB077)
作者简介
通讯作者Tel：0576—86207747；E—mail：chenhongleiwh@126．com
