摘要
采用气相色谱-质谱联用技术(GC-MS)对安徽安庆、浙江金华、河南嵩县和河南驻马店4个产地石菖蒲的挥发性成分进行了研究,共鉴定出45种化学成分,并测定了其相对含量。其中,相对含量大于1%的共有挥发性成分为β-细辛醚、γ-细辛醚、α-细辛醚和顺式-甲基异丁香酚。这4种共有挥发性成分的保留时间及相对含量可作为石菖蒲鉴别和质量评价的必要条件。采用"分区"法,将石菖蒲总离子流色谱图分为4个区,并用相对保留值α和相对峰面积Sr进行比较。结果表明,第Ⅲ区为石菖蒲挥发性成分的主要药效成分区。该区包括28、30和36号色谱峰,对应的挥发性成分分别为γ-细辛醚、β-细辛醚和α-细辛醚。该研究为不同产地石菖蒲的挥发性成分的比较及质量评价提供了新途径。
The dry rhizome of Acorus tatarinowii Schott is a common traditional Chinese medicine and could be used for treatment of lots of diseases, such as epilepsy. In this paper, the volatile constituents of A. tatarinowii Schott from 4 growing areas, e. g. Anqing, Anhui; Jinhua, Zhejiang; Songxian, Henan and Zhumadian, Henan, were investigated by gas chromatography - mass spectrometry （GC- MS）. Total of 45 volatile constituents were tentatively identified and their relative contents were determined. There were 4 common volatile constituents（β-asarone, γ-asarone, α-asarone and cis-methylisoeugenol） in A. tatarinowii Schott, which occupied more than 1% of the contents of the volatile constituents. Their retention time and relative contents could be regarded as the essential con- dition of the identification and quality assessment on A. tatarinowii Schott. In application of the dividing sections, the total ion current chromatograms of A. tatarinowii Schott were divided into 4 sections and were further compared with the relative retention value （a） and the relative peak area （Sr）. It was shown that the 3rd section of the total ion current chromatograms was the major active ingredient of A. tatarinowii Schott and consisted of the 28th, 30th and 36th peaks which corresponded to γ-asa- rone, β-asarone and α-asarone, respectively. This study provided a new approach for the comparison and quality assessment of A. tatarinowii Schott from different growing areas.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第4期407-412,417,共7页
Journal of Instrumental Analysis
基金
广东省科技计划重大专项资助项目(A301020101)
广东省自然科学基金资助项目(01142)
关键词
石菖蒲
气相色谱-质谱联用
挥发性成分
分区
Acorus tatarinowii Schott
gas chromatography-mass spectrometry
volatile constituents
dividing sections
作者简介
通讯作者：曾志，Tel：020—39310068，E—mail：zhizeng@scnu．edu．cn
