摘要
目的:建立天然牛黄HPLC-DAD指纹图谱并与酶促牛黄指纹图谱进行比较。方法:采用高效液相色谱法,色谱条件:DiamonsilC18柱(250 mm×4.6 mm,5μm);流动相为甲醇-氯仿-1%的醋酸水溶液,梯度洗脱;检测波长370 nm;流速1.0 mL/min;分析时间40 min。结果:在选定的色谱条件下建立了天然牛黄色谱指纹图谱共有模式,并对酶促牛黄与天然牛黄的指纹图谱进行比较,两者指纹基本一致,但各成分间的相对含量有差异。结论:所建立的天然牛黄色谱指纹图谱方法精密度高、重现性好,可用于各种牛黄药材替代品的鉴别研究。
AIM：To develop an HPLC-PDA fingerprint analysis method of natural Calculus bovis in comparison with Calculus bovis cultivated by glucuronidase.METHODS：An HPLC analysis was carried out on a Diamonsil C18 column（4.6 mm ×250 mm,5 μm）with the mobile phase consisting Methanol（A）-chloroform-1% acetic acid aqueous solution in gradient mode.The detection wavelength was set at 370 nm.The flow rate was 1.0 mL.min-1 and the analytic time was 40 min.RESULTS：Under the above chromatographic conditions,the mutual mode of HPLC fingerprints was set up,Calculus bovis cultivated by glucuronidase had a similar fingerprint chromatogram to the natral Calculus bovis,but there was a little difference in their concent.CONCLUSION：The method is in good precision and reproducibility and could be available for the identification of various substitutes of Calculus bovis.
出处
《中成药》
CAS
CSCD
北大核心
2011年第1期1-5,共5页
Chinese Traditional Patent Medicine
基金
泰山医学院青年科学基金项目(2009ZRQN060)
山东省教育厅基础项目(J10LF-16)
关键词
天然牛黄
酶促牛黄
高效液相色谱法
指纹图谱
natural Calculus bovis； calculus bovis cultivated by glucuronidase； HPLC； fingerprint；
作者简介
李珂（1978-），女，讲师，硕士。从事药物分析教学及科研工作。Tel：（0538）6229752-8004 E-mail：likegaofeng@126．com
通讯作者：高允生（1955-），男，教授，硕士生导师。Tel（0538）-6229756 E-mail：ysgao@tsmc．edu．cn．
