摘要
目的建立胆红素的含量测定方法，比较3种牛黄中胆红素的含量。方法色谱条件：Diamonsil迪马C18（250mm×4．6mm，5μm）色谱柱；流动相：甲醇-三氯甲烷-1％醋酸（65：32：3），流速：1．0mL／min；检测波长455nm。结果胆红素浓度在2．32～27．84μg／m1范围，峰面积与浓度呈良好的线性关系A=19169C-4886．9（r=0．9998）；平均回收率为99．38％，相对标准偏差值（RSD）=2．16％。结论该法简便、准确、专属强，可用于牛黄、牛黄替代品及其制剂的质量控制。
Objective To establish a HPLC method for determination of bilirubin, and to compare the content of 3 kinds of bezoar. Methods The C18（250 mm ×4.6 mm, 5 μm） column was used. The mobile phase was Methanol-chloroform-1% acetic acid （65 ： 32：3 ）. The flow rate was 1.0 ml/min, and the detection wavelength was 455 nm. Results The bilirubin was in a good linear relationship （ r = 0. 9998 ） at the concentration of 2.32 - 27.84μg/ml. The average recovery was 99.38%, and RSD was 2.16%. Conclusion The method was simple, accurate, specific, and can be used for the quality control of bilirubin in calculus bovis, succedaneums and preparation of calculus bovis.
出处
《今日药学》
CAS
2010年第3期17-19,共3页
Pharmacy Today
基金
泰山医学院青年科学基金项目(编号:2009ZRQN060)
关键词
天然牛黄
酶促牛黄
人工牛黄
胆红素
含量测定
高效液相色谱法
Natural Calculus Bovis
Calculus Bovis Cultivated by Glucuronidase
Atificail Calculus Bovis
Bilirubin
Determination
HPLC
作者简介
李珂，讲师，Tel：0538—6229752—8004，E—mail：likegaofeng@126．com．
