摘要
雷公藤生物碱是雷公藤Tripterygium wilfordii Hook中的主要杀虫活性物质。为进一步阐明雷公藤总生物碱的杀虫活性及杀虫作用机理提供线索和依据,研究采用载毒叶片法测定了雷公藤总生物碱对粘虫(Mythimna separata(Walker))三龄幼虫的胃毒毒力,观察了粘虫五龄幼虫的中毒症状,并测定了其对不同中毒阶段粘虫五龄幼虫头部乙酰胆碱酯酶(AChE)活性和乙酰胆碱(ACh)含量的影响。结果表明,雷公藤总生物碱对粘虫三龄幼虫48,72,96,120 h的胃毒LC50分别为306.39,216.12,157.18,129.92 mg/L;试虫中毒后行动迟缓,停止取食,继而软瘫麻痹(即麻醉),后复苏(或死亡),复苏试虫可再次取食、麻醉、复苏(或死亡),如此反复,直止死亡;雷公藤总生物碱对各中毒期试虫AChE无明显的抑制或激活作用;麻醉期试虫体内ACh的相对含量与同期对照无显著差异,复苏期有所下降。
Alkaloid is the main insecticidal composition in Tripterygium wilfordii Hook,a famous insecticidal plant.In order to study its insecticidal activites and mechanism,the stomach toxicity of the total alkaloid against larvae of Mythimna separata Walker was tested,their toxicity symptoms were observed,and its effects on the activities of AChE and the content of ACh of larval heads in different toxicity stages were assayed.The results showed the stomach toxicity LD50 values of the total alkaloid against the 3rd instar larvae of M.separata in 24,48,96 and 120 h post treatment were 306.39,216.12,157.18 and 129.92 mg/L.In vivo toxicity symptoms were characterized by a definite sequence of events,starting with moving slowly,stopping feeding,flaccid paralysis and recovering or death.Anabiotic insects started feeding,were paralyzed,recovered or died again and up to death.It was presumed the total alkaloid from T.wilfordii influenced the nerve system of insects.AChE was not strongly inhibited or activated by the total alkaloid.ACh content in paralysis stage wasn′t significantly different with that of the control and lower in recovery stage.
出处
《华北农学报》
CSCD
北大核心
2011年第1期215-218,共4页
Acta Agriculturae Boreali-Sinica
基金
河南省科技攻关项目(082102350033)
关键词
雷公藤
总生物碱
粘虫
中毒症状
乙酰胆碱酯酶
乙酰胆碱
Tripterygium wilfordii Hook
Total alkaloid
Mythimna separata Walker
Toxicity symptom
AchE
ACh
作者简介
周琳（1971-），女，河南南阳人，副教授，博士，主要从事生物农药及植物保护研究。
