摘要
采用彗星电泳技术,比较研究了Aroclor 1254、BaP、DDT三种典型POPs对紫贻贝(Mytilus edulis)鳃、性腺、消化腺细胞的DNA损伤效应的差异,以期为进一步评价这三种典型POPs遗传毒理机制及其预警监测提供科学依据。结果表明,Tail DNA%是评价DNA损伤的理想指标,另外,在急性毒性试验中,这三种POPs对紫贻贝鳃、性腺、消化腺细胞的DNA损伤作用均存在显著时间-剂量-效应关系,其中,BaP的DNA损伤作用最大,鳃和消化腺细胞对毒性作用较敏感。同时,对不同组织细胞的敏感性差异原因及其在环境监测中的应用进行了探讨。
In this article,we compared the DNA damage in blue mussel gill,gonad and digestive gland cells that were exposed to Aroclor1254、BaP and DDT by comet assay.The results show that tail DNA% was a perfect parameter for DNA damage.In addition,there were significant time-and dose-dependent damage to the gill,gonad and digestive gland cells of blue mussel.Furthermore,the toxic effect of BaP was much more than the other two POPs;and gill and digestive gland cells were much more sensitive than gonad cells for the POPs toxicity.We also discussed the reason underlining the toxic sensitivity of different tissues and the significance of monitoring POPs in marine ecosystem.The results are helpful for assessing the toxic effects and exploring the toxicological mechanism of POPs.
出处
《海洋科学》
CAS
CSCD
北大核心
2011年第2期32-37,共6页
Marine Sciences
基金
中国科学院重大项目(KZCX2-YW-Q07-05
KZCX2-YW-Q07-04)
作者简介
周海龙（1977-），重庆人，博士，讲师，研究方向为海洋环境毒理学，电话：0898—66180255，E-mail：hlongzhou@gmail.com；
薛钦昭，通信作者，0535-2109020；E-mail：qzxue@yic．ac.cn
