摘要
以栉孔扇贝(Chlamys farreri)鳃和消化盲囊中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量为指标,探讨不同浓度(对照、0.18、0.32和0.51mg.L-1)原油水溶性成分(WSF)在不同时间(1、2、4、7、10d)对栉孔扇贝的毒性效应。结果表明,WSF对扇贝鳃和消化盲囊中SOD和CAT活性均有不同程度的诱导效应,酶活性达到最高的时间随WSF浓度的不同而变化。各实验组鳃中MDA含量自第4天开始均高于对照组;0.18mg.L-1浓度组消化盲囊中MDA含量低于对照组,0.32和0.51mg.L-1浓度组消化盲囊中MDA含量一般高于对照组。进一步分析显示,第2～10天消化盲囊中SOD活性与WSF浓度存在显著的正相关性,表明消化盲囊中SOD活性更可能作为石油污染的有效生物标志物。
The effects of water-soluble fraction （WSF） of crude oil on the activity of antioxidant enzymes and lipid peroxidation in the gill and digestive gland of Chlamys Ferrari were investigated. Chlamys Ferrari was exposed to WSF at different concentrations （0.02, the control, 0. 18, 0. 32 and 0. 51 mg · L-1） for 10 days. The results indicated that the activities of superoxide dismutase （SOD） and catalase （CAT） in gill and digestive gland were induced by WSF in various concentrations to different levels, and the time for the enzymes reaching their highest activity varied with the concentration of WSF. The contents of malond- ialdehyde （MDA） in the gill of all test groups were higher than that in control after being exposure for 4 days. The content of MDA in digestive gland of 0. 18 mg L-1 group remained lower than that of control; while the contents of MDA in both the 0. 32 and 0.51 mg L-1 groups were generally higher than that of control. In addition, activity of SOD in digestive gland was positive correlated with the concentration of WSF from day 2 to day 10, indicating that the effect of petroleum hydrocarbons on the activity of SOD in digestive gland was a good indicator of petroleum hydrocarbons pollution.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第7期45-50,共6页
Periodical of Ocean University of China
基金
海洋公益性课题项目(200705011)
中国海监总队业务化项目(201105013)
国家海洋局第一海洋研究所基本科研业务专项资金项目(2010T04)
国家海洋局青年海洋科学基金项目(2012534)资助
关键词
原油水溶性成分
栉孔扇贝
抗氧化酶
脂质过氧化
water-soluble fraction of crude oil Chlamys farreri antioxidant enzyme lipid peroxidation
作者简介
王晓艳（1987-），女，硕士生。E-mail：xiaozhu11290@126．com
通讯作者：E-mail：jiangfh@rio．org．cn
