摘要
以玉米提取淀粉后的玉米皮渣为主要原料,采用黑曲霉固态发酵法产酶再酶解的二步法降解玉米皮中纤维素类物质。经Plackett-Burman法及响应面设计优化发酵条件得:温度30℃,接种量10%,初始水分体积分数60%,物料厚度2.47 cm,初始pH 5.79,发酵时间6 d;滤纸比酶活可达11.01 U/g,较原始酶活提高了40.61%;产酶结束后加入pH 4.8醋酸-醋酸钠缓冲液,置于50℃下酶解144 h,中性洗涤纤维与酸性洗涤纤维降解率分别为46.09%、48.82%,还原糖质量分数达到9.02%。
The residues of corn bran were used as main substrates for solid-state fermentation（SSF） and the cellulose in corn bran was degraded.The first step was cellulase production with Aspergillus Niger by SSF and the second step was enzymatic degradation of cellulose in corn bran.The Plackett-Burman and Response Surface Methods were used for optimization.The fermentation conditions were as follows：temperature 30 ℃,10% inoculum,initial moisture content of 60%,material thickness of 2.47 cm,pH of 5.79,fermentation time 6 d.The filter paper activity was 11.01 U/g and increased 40.61% higher than the original enzyme activity.pH 4.8 acetate-sodium acetate buffer solution was added after fermentation and enzymolysis at 50 ℃ for 144 h,neutral detergent fiber and acid detergent fiber degradation rates were 46.09% and 48.82 %,respectively,and the reducing sugar content was 9.02%.
出处
《生物加工过程》
CAS
CSCD
2011年第2期39-43,共5页
Chinese Journal of Bioprocess Engineering
关键词
黑曲霉
纤维素
玉米皮
降解
固态发酵
Aspergillus niger
cellulose
corn bran
degradation
solid-state fermentation
作者简介
胡慧东（1985-），男，山西吕梁人，硕士研究生，研究方向：微生物固态发酵；
许赣荣（联系人），教授，E-mail：grxul23@126．com
