摘要
[目的]了解江苏省外环境中霍乱弧菌的污染现状,探讨其与病例发生、疾病流行间的关系。[方法]检测水体、水产品和熟食中霍乱弧菌的携带情况,比较外环境来源株与病例株在血清型、耐药性及毒素基因等方面的差异,应用PFGE分型技术分析不同来源菌株的遗传相关性。[结果]3株外环境来源株均为不带毒力、对抗生素敏感的O1群非流行株,病人来源株为具有ctxA、zot、ace、tcpA毒力基因的O139群。11株O139群被分成7种PFGE带型,与O1群相似性系数为76.14%。[结论]外环境来源株在表型及基因水平上与病例株差异较大,不是病例发生的原因。目前霍乱在江苏省处于低频的高度散发状态,近期大规模暴发和流行的可能性较小。
[Objective] To explore the environmental vibrio cholerae pollution situation and its correlation with the occurrence and epidemic of cholera in Jiangsu province. [Methods] Examined vibrio cholerae from water, aquatic products and cooked food. Compared serum type, drug-resistance and virulence genes between strains from environment and patients. Analyzed the genetical correlation among strains from different resources by PFGE. [Results] 3 isolates from environment were non-epidemic strains without virulence genes and not sensitive to antibiotics. The strains from patients were O139 with toxic genes ctxA, zot, ace, tcpA. 11 strains of O139 were classified into 7 PFGE types, and the similarity coefficient was 76.14% with O1 strains. [Conclusion] The strains from environment are different from those from patients on the level of phenotype and gene. The cases are not caused by the strains from environment. At present, the epidemic intensity of cholera in Jiangsu province is low and highly sporadic, and the chance of outbreak and prevalent is small.
出处
《现代预防医学》
CAS
北大核心
2011年第3期430-431,434,共3页
Modern Preventive Medicine
作者简介
庄菱（1973-）．女．硕士，副主任技师．研究方向：肠道传染病防制
