摘要
目的:探讨外周血B淋巴细胞活化因子(BAFF)和B淋巴细胞活化因子受体(BAFF-R)之间的相关性以及两者与I型自身免疫性肝炎(AIH-I)患者临床特征的关系.方法:检测30例AIH-I患者治疗前后外周血BAFF(酶联免疫吸附法)及BAFF-R(流式细胞仪)的表达水平和30名健康志愿者外周血中二者的表达水平,同时收集实验对象相应的IgG、ALT、AST、GLO、CRP检查结果,通过SPSS统计软件进行分析.结果:AIH-I患者外周血BAFF及BAFF-R水平较正常对照组有统计学差异(747.83ngL±66.96ngLvs203.50ngL±43.99ngL;18.78%±5.08%vs1.98%±0.89%,均P<0.05);AIH-I患者外周血BAFF与BAFF-R水平呈正相关(P<0.05);AIH-I患者外周血BAFF及BAFF-R水平在治疗前后有统计学差异(747.83ngL±66.96ngLvs582.83ngL±51.89ngL;18.78%±5.08%vs9.93%±3.53%,均P<0.05);AIH-I患者外周血BAFF及BAFF-R水平与疾病活动指标(IgG、ALT、AST、GLO、CRP)水平无明显相关(均P>0.05).结论:AIH-I患者外周血BAFF与BAFF-R水平明显升高,激素治疗前后差异显著,二者与疾病的发展过程相关,对评价疾病的治疗具有一定的指导意义.
AIM： To detect serum level of B-lymphocyte activating factor （BAFF） and percentage of B cells positive for B-cell activating factor receptor （BAFF-R） on B cells in patients with type I autoimmune hepatitis （AIH-I） and to analyze their relations to the clinical features of AIH-I. METHODS： Thirty patients with AIH-I and 30 healthy subjects were included in the study. Serum BAFF was detected by enzyme-linked immunosorbent assay （ELISA）, and the percentage of BAFF-R-positive B cells was measured by flow cytometry. Meanwhile, serum levels of AL T, AST, GLO, CRP and IgG were measured. RESULTS： There were significant differences in mean serum BAFF level and percentage of BAFF-R-positive B cells between AIH-I patients and healthy subjects （747.83 ng L ± 66.96 ngL vs 203.50 ngL ± 43.99 ngL; 18.78% ± 5.08% vs 1.98% ± 0.89%, both P 0.05）. There is a positive correlation between serum BAFF level and percentage of BAFF-R-positive B cells in AIH-I patients （P 0.05）. Significant differences were noted in mean serum BAFF and percentage of BAFF-R-positive B cells in AIH-I patients between before and after treatment （BAFF： 747.83 ngL ± 66.96 ngL vs 582.83 ngL ± 51.89 ngL, P 0.05; BAFF-R： 18.78% ± 5.08% vs 9.93% ± 3.53%, P 0.05）. Serum BAFF level and percentage of BAFF-R-positive B cells showed no correlation with serum ALT, AST, GLO, CRP, or IgG in AIH-I patients （all P 0.05）. CONCLUSION： The level of serum BAFF and percentage of BAFF-R-positive B cells are significantly increased in AIH-I patients but significantly descended after treatment.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第1期89-93,共5页
World Chinese Journal of Digestology
作者简介
通讯作者：徐芸，教授，450052，河南省郑州市，郑州大学第一附属医院消化内科．xuyun@medmail.com.cn电话：0371-66295922
