摘要
目的 建立以流式细胞仪检测人ABO天然血型特异性抗体的技术,并检验其效果.方法 将52份健康志愿者的血清样本与标准化人源红细胞混合,加入异硫氰基荧光素标记的羊抗人IgM或IgG二抗,用流式细胞仪检测样本中抗红细胞抗原A和B的特异性IgM及IgG抗体.检测结果与酶标仪法进行对比.结果两种方法对于血型IgM抗体水平变化趋势检测的关联性较好,二者的Spearman等级相关系数为0.730（P＜0.05）,但流式细胞术的特异性、灵敏性和重复性优于酶标仪法,A型和B型血清样本（各16例）中,流式细胞术结果转为阴性的IgM抗体滴度为1∶1024～1∶512,而酶标仪法则为1∶64～1∶32.用流式细胞术在O型血清中检测到IgG抗体.结论 流式细胞术可以进行ABO血型IgM和IgG抗体的检测,效果优于传统方法,并可半定量检测抗体水平.
Objective To establish and validate a standard method using flow cytometry （FCM）in human ABO blood group antibody （Ab） detection. Methods Sera samples from 52 blood donors were incubated with standardize red blood cells （RBCs）, and anti-A/B antibody （IgM/IgG） was measured by indirect flow cytometry after adding secondary isotype-specific fluoresce labeled Ab. The results were compared with those by using ELISA reader. Results The changes of anti-A/B IgM measured by both methods correlated well and an overall correlation coefficiency of 0.730 for IgM was obtained by Spearman＇s rank testing （P〈0.05）. FCM showed better specificity, sensitivity and reproducibility over ELISA reader. In blood group A samples （n = 16） or B samples （n = 16） ,the antirespectively. IgG blood group Ab could only be detected in blood group O sera. Conclusion Compared with traditional methods,FCM is a more objective method to offer accurate detection of natural ABO blood group Ab （IgM/IgG） and allowed semi-quantitative measurement of antibody levels.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2011年第1期24-27,共4页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金
作者简介
通信作者：陈刚，Bmail：gchen@tjh.tjmu.edu.cn
