摘要
目的:建立测定复方红景天口含片中红景天苷和酪醇含量的方法。方法:采用高效液相色谱法。色谱柱为AgilentTC-C18(250mm×4.6mm,5μm),流动相为乙腈-水(8.5∶91.5),流速为1.0mL·min-1,检测波长为275nm。结果:红景天苷、酪醇的进样量分别在0.642～5.136μg(r=0.9997)、3.600～36.000μg(r=0.9991)范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为100.29%、99.46%,RSD分别为1.69%、2.25%(n=9)。结论:本方法简便、快速、重复性好,可用于复方红景天口含片的质量控制。
OBJECTIVE： To establish a method for the content determination of rhodioloside and tryosol in Compound rhodiola buccal tablets. METHODS：The content of rhodioloside and tryosol were deteminationed by HPLC. A Agilent TC-C18 column （250 mm ×4.6 mm, 5 μm） was used, the mobile phase was consisted of acetonitrile-water （8.5：91.5） . The flow rate was 1.0 mL. min 1, and detection wavelength was set at 275 nm. RESULTS： The linear range of rhodioloside was 0.64245.136μg（r=0.999 7） with an mean recovery of 100.29% （RSD=1.69%, n=9）. The linear range of tryosol was 3.600-36.00μg（r=0.999 1） with an mean recovery of 99.46 % （RSD = 2.25 %, n = 9）. CONCLUSION： The method is simple, rapid and reproducible for the quality control of Compound rhodiola buccal tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第7期621-622,共2页
China Pharmacy
基金
兰州市科技计划项目(2008-1-177)
作者简介
硕士研究生。研究方向：中药化学及中药质量标准。E-mail：songwei_0112@sina．com
通讯作者：主任药师，硕士研究生导师。研究方向：天然药物化学及中药制剂。电话：0931-2614521-6039。E-mail：shixiaofeng2005@sina．com
