摘要
目的：研究内生真菌诱导子对茅苍术细胞生长以及苍术素积累的影响。方法：采用细胞悬浮培养方法研究内生真菌诱导子不同浓度、不同加入时间对茅苍术细胞生长、苍术素合成以及相关酶活性的影响。结果：低浓度诱导子对细胞生长没有明显的影响,但高浓度诱导子显著抑制细胞生长,当诱导子质量浓度达到100 mg.L-1时,细胞生长抑制率达到46.7%;此外,从茅苍术内生真菌中筛选获得6株具有较强诱导作用的内生真菌,其中Rhizoctonia SP1诱导子的作用较强,2060 mg.L-1Rhizoctonia SP1诱导子显著促进苍术素的生物合成。在细胞培养至12 d时,添加40 mg.L-1Rhizoctonia SP1诱导子,苍术素的含量在第21天达到最大值28.06μg.L-1,比对照提高了48.3%;且细胞多酚氧化酶（PPO）、过氧化物酶（POD）、过氧化氢酶（CAT）的活性出现了显著提高。结论：内生真菌诱导子能有效提高茅苍术细胞悬浮培养体系中苍术素的产量。
Objective： To investigate the effects of endophytic fungal elicitors on the growth and atractylodin accumulation of cell suspension cultures of Atractylodes lancea.Method： The endophytic fungal elicitors were added to the medium with different concentrations and culture period.Their effects on biomass,atractylodin content and relevant enzyme activities in suspension cultured cells were studied.Result： The cell growth was not affected by elicitors at low concentration and obviously inhibited at high concentration.Inhibition rate reached 46.7% by 100 mg·L-1 elicitor.In addition,six strains from A.lancea,among which Rhizoctonia SP1 activity was higher,had distinctly promoted the accumulation of atractylodin.Atractylodin biosynthesis was notably promoted by 20-60 mg·L-1 Rhizoctonia SP1 elicitor.When 40 mg·L-1 Rhizoctonia SP1 elicitor was added in the medium at the 12 day,the maximum content of atractylodin was 28.06 μg·L-1 at the 21 day with 48.3% higher than that of the control and PPO,POD and CAT activities remarkably increased.Conclusion： Adding the endophytic elicitors to the medium is able to be effective approaches to enhance atractylodin yield in the suspension culture cell of A.lancea.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第1期27-31,共5页
China Journal of Chinese Materia Medica
基金
江苏省高校自然科学基础研究项目(07KJD360164)
江苏省中医药局项目(HL07076)
关键词
内生真菌
诱导子
茅苍术
细胞生长
苍术素
endophytic fungi
elicitor
Atractylodes lancea
cell growth
atractylodin
作者简介
[通信作者]陶金华，硕士，主要从事中药生物技术研究，E-mail：taojinhua2000@163．com
