摘要
在壳聚糖膜的表面接上分子链较长的双醛淀粉作为柔性固定化酶的载体,将木瓜蛋白酶固定在该载体上制成柔性固定化木瓜蛋白酶,并应用于酶解波纹巴非蛤制备小分子肽。通过考察温度、pH值、加酶量和固定化时间等条件对柔性固定化木瓜蛋白酶的酶活、酶活回收率的影响,确定最优固定化条件。结果表明:双醛淀粉用量0.8mg/g壳聚糖、柔性固定化酶温度25℃、柔性固定化酶时间20h、加酶量40mg/g壳聚糖、pH8.0条件下所得的固定化酶酶活最高,酶活回收率63.35%。将该柔性固定化酶在酶解温度40℃、pH7.0、加酶量1.0%、酶解时间4h条件下制备波纹巴非蛤小分子肽,产率为3.4055%。
Long-chain dialdehyde starch was linked to the surface of chitosan membrane to obtain an immobilization carrier for enzyme. Flexible immobilized papain was prepared through fixing papain on the carrier and applied to prepare small molecule peptide by hydrolyzing aphia undulate. The optimal immobilization conditions were explored by evaluating the effect of immobilization temperature, pH, enzyme amount and immobilization time on enzyme activity and recovery rate of flexible immobilized papain. Results indicated that the optimal immobilization conditions were reaction temperature of 25 ℃, reaction time of 20 h, enzyme amount of 40 mg/g, and pH 8.0. Under the optimal immobilization conditions, the obtained immobilized papain exhibited the highest activity and the recovery rate of papain was 63.35%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第22期500-503,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2008BAD94B08)
中国科学院知识创新工程重要方向项目(KZCX2-YW-209)
广东省2009中国科学院-广东省合作项目(00617381220824077)
关键词
壳聚糖
双醛淀粉
固定化酶
chitosan
dialdehyde starch
immobilized enzyme
作者简介
陈忻（1968-），女，副教授，硕士，主要从事生物材料及海洋化学研究。E-mail：fschenxin@tom．com
