摘要
本研究采用乙醇-硫酸铵双水相体系协同超声提取法分离金银花中的总黄酮,通过单因素实验和正交试验考察了提取时间、料液比、醇水比和分相盐浓度对总黄酮提取率的影响。实验结果表明:按醇水比0.7(V/V),硫酸铵浓度为0.27g/mL配制双水相,在料液比1:25(m/V),50℃温度下超声提取75min,总黄酮提取率为1.97%。
Total flavonoids from honeysuckle flower was separated by alcohol-ammonium sulfate two-phase aqueous system synergism with ultrasound extraction.Influence of extraction time,material-solvent ratio,ethanol-water ratio and salt concentration on extraction ratio of total flavonoids was researched by single factor experiment and orthogonal test.The results showed that using ethanol-water ratio for 0.7（V/V）,ammonium sulfate concentration for 0.27g/mL preparation two-phase aqueous solution,extraction yield of total flavonoids is 1.97% in material-solvent ratio for 1：25（m/V）,60℃ the ultrasonic extraction 75min.
出处
《食品与发酵科技》
CAS
2010年第6期93-96,共4页
Food and Fermentation Science & Technology
基金
湖南省科技厅科技计划项目(编号:2008FJ3077)
2010年湖南省大学生创新性实验计划项目(湘教通【2010】244号)
关键词
双水相
超声提取
金银花
总黄酮
two-phase aqueous system
ultrasonic
honeysuckle flower
total flavonoids.
作者简介
作者简介：许灵君（1988-），男，本科生，专业方向：天然产物提取与食品添加剂。
通讯作者：欧阳玉祝，男，教授，硕士生导师。
