摘要
将转RD29A-CBF3基因烟草幼苗及对照(未转基因烟草)在15℃和4℃低温胁迫8h后,测定生理生化指标。结果表明,SOD活性、POD活性、MDA含量以及游离脯氨酸含量均增加,4℃处理比15℃处理增加的幅度大;转基因烟草的SOD活性、POD活性、MDA含量以及游离脯氨酸含量与对照均存在极显著的差异(P<0.01),说明转基因烟草幼苗获得了一定的抗寒能力。
The RD29A-CBF3 transgenic seedlings and the control（Normal tobacco seedlings） were treated at 15℃ and 4℃ for 8 h,and the physiological and biochemical indexes were determined.The results showed that SOD activity,POD activity,MDA content and free proline content in transgenic tobacco and the control were all increased,and the increasing extents under 4℃ stress were higher than that under 15℃ stress;There existed significant deviation（P 0.01） between transgenic and normal tobacco plants in SOD activity,POD activity,MDA content and free proline content.The results demonstrated that the transgenic tobacco seedlings obtained the cold resistance.
出处
《湖北农业科学》
北大核心
2010年第11期2819-2821,共3页
Hubei Agricultural Sciences
作者简介
作者简介：刘荣梅（1975-），女，黑龙江哈尔滨人，讲师，博士，从事植物分子生物学研究，（电话）15246641710（电子信箱）lrm1975@sina.com.
通讯作者，胡宝忠，教授，主要从事植物生殖生物学与分子生物学研究，（电话）0451-55190866（电子信箱）bzhu@neau．edu．cn。
