摘要
目的研究紫杉醇脂质体体外对人直肠癌Colo320细胞的影响。方法以0.01、0.1、1、10、100μmol/L等浓度梯度的紫杉醇脂质体作用于人直肠癌Colo320细胞,并设立空白对照组,通过MTT法和TUNEL法等技术检测和分析紫杉醇脂质体药对Colo320细胞增殖和凋亡的影响。结果上述不同浓度的紫杉醇脂质体作用于直肠癌Colo320细胞24～96h,随着作用时间的延长和药物浓度的升高,细胞存活率逐渐降低,作用于Colo320细胞24h,随着药物浓度的升高,细胞凋亡指数逐渐升高。结论紫杉醇脂质体体外可以抑制人直肠癌Colo320细胞的增殖,促进细胞的凋亡。
Objective To study effect of paclitaxel liposome on the human rectal cancer cell Colo320 in vitro. Methods The effects of 0. 01 μmol/L,0. 1 μmol./L,1 μmol/L,10 μmol/L,100 μmol/L and blank on Colo320 cell were measured by MrI＇T and TUNEL. To analyze the effects of cell proliferation and apoptosis about paclitaxel liposome on the human rectal cancer cell Colo320. Results Cell survival rate degrade gradually by lengthening action time of paclitaxel liposome on Coto320 cell or heightening density of paclitaxel liposome. Apoptotic index is heightened gradually by heightening medicine density in 24 hours of action. Conclusion Paelitaxel liposome can restrain cell proliferation of the human rectal cancer cell Colo320 and promote apoptosis in vitro.
出处
《中国实用医药》
2010年第31期13-14,共2页
China Practical Medicine
关键词
紫杉醇脂质体
直肠癌
细胞增殖
细胞凋亡
Paclitaxet liposome
Rectal cancer
Cell proliferation
Apoptosis
作者简介
通讯作者：张红雨Email：baojunlv@163．com
