摘要
为观察牛主动脉中蛋白聚糖对培养的人脐静脉内皮细胞生长的影响及其在动脉粥样硬化形成中的作用，用解聚提取、离子交换及凝胶过滤柱层析法从牛主动脉内、中膜分离出硫酸乙酰肝素蛋白聚糖、硫酸软骨素蛋白聚糖和硫酸皮肤素－硫酸软骨素蛋白聚糖。用氚标胸腺嘧啶脱氧核苷掺入法和细胞计数法观察上述三种蛋白聚糖对培养的第一代人脐静脉内皮细胞生长的影响。结果发现，用氚标胸腺嘧啶脱氧核苷掺入法计算硫酸乙酰肝素蛋白聚糖的抑制率（醛酸含量２．１～８．３ｍｇ／Ｌ）分别为２８．３％、５２．３％和７５．４％，硫酸软骨素蛋白聚糖的抑制率（醛酸含量９．５～３７．８ｍｇ／Ｌ）分别为６１％、８３％和９１％，硫酸皮肤素－硫酸软骨素蛋白聚糖的抑制率（醛酸含量９．３～３６．９ｍｇ／Ｌ）分别为５８．４％、８２．４％和８６％。用细胞计数法计算硫酸乙酰肝素蛋白聚糖（醛酸含量２．１～８．３ｍｇ／Ｌ）的抑制率分别为２１．５％、３７．８％、６２．９％，硫酸软骨素蛋白聚糖（醛酸含量９．５～３７．８ｍｇ／Ｌ）的抑制率分别为３７．２％、６８．９％和７６．５％，硫酸皮肤素硫酸软骨素蛋白聚糖（醛酸含量９．３～３６．９ｍｇ／Ｌ）的抑制率分别为４４．５％、６１．６％和７１．７％。结果?
Aim To investigate the effect of bovine aortic heparan sulfate proteoglycan (HSPG) ,chondroitin sulfate proteoglycan(CSPG) and dermatan chondroitin sulfate proteoglycan (DSCSPG) on the growth of cultured human umbilical vein endothelial cells (hUVEC). Methods PGs were isolated by dissociative extraction and column chromatography from bovine aortic intima media and their effects on the growth of cultured first passaged hUVEC was studied by 3 H TdR incorporation and cell count. The first passaged hUVEC (2.5×10 4) were planted onto 24 well plant (5 wells or 3 wells for each PG) and cultured by M 199 containing 15% HS and 10% FCS for 24 h, and then the hUVEC were incubated with M 199 containing 15%HS, 10%FCS and different concentrations of PGs (PGs were presented as hexuronic acid) respectively together with controls without PG for 24 h. Results The inhibiting percentages of proliferation (based on 3H TdR incorporation and cell count ) with HSPG (hexuronic acid 2.1～8.3 mg/L) were 28.3%、52.3% and 75.4%(21.5%、37.8% and 62.9%), with CSPG (hexuronic acid 9.5～37.8 mg/L) were 61%、83% and 91%(37.2%、68.9% and 76.5%) and with DSCSPG (hexuronic acid 9.3～36.9 mg/L) were 58.4%、82.4% and 86%(44.5%、61.6% and 71.7%) Conclusion bovine aortic HSPG, CSPG and DSCSPG could inhibit the proliferation of hUVEC and the inhibitions were associated with the PG concentrations.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期158-160,共3页
Chinese Journal of Arteriosclerosis
关键词
蛋白聚糖
血管内皮细胞
动脉粥样硬化
主动脉
Proteoglycans
Heparitin Sulfate
Chondroitin Sulfates
Dermatan Sulfate
Muscle, Smooth, Vascular
Cell Division
Growth Inhibitors
Aorta
