摘要
优化了尖孢镰刀菌液体发酵生产蒽醌类红色素的发酵条件。通过单因素实验和正交优化实验,确定最佳产色素发酵培养基为:可溶性淀粉30%,(NH4)2SO43%,MgSO40.3%,KH2PO44%,pH6.0。产色素最适培养条件为:初始pH6.0,装液量20%,接种量10%,吐温-80添加量1%,温度28℃,摇床转速200r/min,发酵周期120h。此条件下,色素效价即可达到8.184U/ml,比优化前提高了1.8倍。国内首次对尖孢镰刀菌所产蒽醌色素进行研究,为其进一步应用奠定基础。
The liquid fermentation conditions for anthraquinone pigment by Fusarium oxysporum was optimized.Single factor experiment and orthogonal optimization experiment determined the optimal fermentation conditions for production as follows：the optimate composition of the medium：soluble starch 30%,（NH4）2SO4 3%,MgSO4 0.3%,KH2PO4 4%,and the optimal fermentation conditions：initial medium pH 6.0,medium volume 20%,inoculation amount 10%,Tween-80 1.0%,incubation temperature 28℃,rotation speed 200r/min and the fermentation period was 120h.In the optimal conditions,the color value reached 8.184U/ml,and increased by about 1.8 folds compared with the unoptimized conditions.It is the first time to study the anthraquinone pigment produced by Fusarium oxysporum at domestic.And it is hoped to lay the foundation for its further application.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2010年第9期56-61,共6页
China Biotechnology
关键词
尖孢镰刀菌
蒽醌色素
色素效价
液体发酵
条件优化
Fusarium oxysporum Anthraquinone pigment Color value Liquid fermentation Optimization
作者简介
电子信箱：fetbcui@seut．edu．cn
