摘要
研究表明淡紫拟青霉次生代谢物质对尖胞镰刀菌的孢子萌发与菌落生长有明显的抑制作用。淡紫拟青霉与镰刀菌活体间存在明显的营养竞争作用;淡紫拟青霉菌能产生细胞壁降解酶和蛋白或多糖性质的拮抗性物质;此活性物质存在于胞内和胞外,胞外物质和胞内提取液-1对尖胞镰刀菌孢子生成的抑制率在90％以上,粗蛋白提取液和粗多糖提取液的抑制率在73％左右,丙酮提取液抑制率最低,仅为25.42％;淡紫拟青霉抑菌活性物质主要存在于水相中;活性物质对温度耐受力较差,100℃或80℃处理30min,基本失去活性。
Studies were conducted to assess the antagonistic effect of biocontrol fungus, Paecilomyces lilacinus （Thorn.） Samson and its mechanism against Fusarium oxysporum Schl. The results showed that the secondary metabolite produced by P. lilacinus obviously suppressed the colony growth, sporulation and germination of F. oxysporum. The antagonistic mechanism against F. oxysporum were mainly related to remarkable nutrient competition and β-glucosidase activity that caused cell wall degradation as well as antibiotic production （such as toxic protein and amylose）, The antibiotic substance existed ＂in the hyphae ,but it could be released from the hyphae significantly in the later stage of ferment culture. The inhibition rates of extracellular substance and intracellular-1 substance to sporeulation of F. oxysporum were above 90%. Meanwhile,the inhibition rates of extractions of crude protein and crude amylose were about 73%, but that of the substance extracted by acetone solvent extraction was only 25.42%. The secondary metabolite mainly being water-solubility and the antibiotic substance was unable to bear high temperature. When treated by 100 ℃ or 80 ℃ for 30 min,the substance had nearly no inhibition activities.
出处
《植物保护学报》
CAS
CSCD
北大核心
2005年第4期373-378,共6页
Journal of Plant Protection
基金
基金项目:国家"863"计划项目(2002AA244031-2)
福建省计委项目[(2003)170]
关键词
淡紫拟青霉
尖孢镰刀菌
拮抗机制
Paecilomyces lilacinus
Fusarium oxysporum
antagonistic mechanism
作者简介
李芳，女，1963年生，副教授，主要从事生物农药研究，email：seapump@126．com
通讯作者，email：fzliubo@163．com
