摘要
用ＫＬＨＥＬＩＳＡ和ＳＥＡＥＬＩＳＡ分别检测日本血吸虫病人治疗前ＩｇＧ及治疗后不同时期血清中特异性ＩｇＧ和ＩｇＧ４。结果表明：两种方法在敏感性和特异性方面差异无显著性（Ｐ＞０．０５）；用ＫＬＨ检测治疗后１２个月血清中ＩｇＧ及ＩｇＧ４的阴转率均显著高于ＳＥＡ者；ＫＬＨ检测治疗后２４个月血清中ＩｇＧ阴转率为９２９％，ＩｇＧ４阴转率为９７６％。提示ＫＬＨ检测特异性ＩｇＧ和ＩｇＧ４具有较高的诊断价值和疗效考核价值；特异性ＩｇＧ４可能系一疗效敏感抗体，可望作为疗效评价指标。
The specific IgG and IgG 4 antibodies in the sera of patients with Schistosoma japonicum were determined by KLH ELISA and SEA ELISA. The results showed that KLH ELISA was as sensitive and specific as SEA ELISA. The negative conversion rates of IgG and IgG 4 12 months after treatment detected by KLH ELISA were significantly higher than those by SEA ELISA. The negative conversion rate of IgG was 92.9%, and that of IgG 4 was 97.6% 24 months after treatment. Therefore, it is regarded that detecting specific IgG and IgG 4 by KLH has high value in both diagnosing schistosomiasis japonica and assessing its therapeutic efficacy. Particularly the specific IgG 4 may be a short duration antibody which can indicate successful chemotherapy of patients with chronic Schistosoma japonicum infection.
出处
《湖南医科大学学报》
CSCD
1999年第3期219-221,共3页
Bulletin of Hunan Medical University
基金
"九五"国家医学科技攻关项目
关键词
日本血吸虫病
钥孔Qi血兰蛋白
IGG
IGG4
Schistosoma japonicum
diagnosis
keyhole limpet haemocyanin
enzyme linked immunosorbent assay
therapy
evaluation studies
