摘要
针对人参根组织中多酚和多糖类物质含量较高的特点,比较了CTAB-LiCl法、pBIOZOL植物提取试剂法、植物RNAout法和优化的Trizol法4种RNA分离提取方法。结果表明:4种方法均能从新鲜的多年生人参根组织中分离提取到总RNA。其中优化的Trizol法能有效地抑制多酚和多糖对总RNA提取的影响,能从成熟的根组织中获得完整性好的总RNA,每克人参根组织RNA产量为100～120μg,OD260/OD280为1.8～2.0,OD260/OD230为2.0～2.3。
Extraction of high-quality RNA from Panax ginseng root tissues is very difficult due to high levels of polyphenolics and polysaccharides that bind or coprecipitate with RNA. The methods such as CTAB-LiCl, pBIOZOL, RNAout and optimized Trizol were applied to isolate high purity and integrity RNA from Panax ginseng root. By all of these methods total RNA can be obtained, but only by the optimized Trizol method, it extracts integrity and high class RNA from mature root tissue. RNA production rate for 100--120 μg/g total RNA from root tissues can be obtained. The absorbance ratios （0D260/ OD280） were 1.8 to 2.0. The absorbance ratios （OD260/OD230） were 2.0 to 2.3.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2010年第5期505-508,522,共5页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(30570185)
关键词
RNA提取
人参
条件优化
RNA extraction
Panax ginseng
condition optimization
作者简介
作者简介：胡薇，女，博士，副教授，研究方向：生物化学与分子生物学。
通讯作者
