摘要
对贡水白柚柚皮乙醇提取物的体外抗氧化作用进行研究,同时与椪柑皮乙醇提取物、VC、没食子酸丙酯(PG)等物质的抗氧化作用进行对比。结果表明:在水杨酸体系中椪柑皮乙醇提取物上清液、VC和PG对羟自由基的清除能力分别是贡水白柚柚皮乙醇提取物上清液的5.515、1.320、0.428倍;而椪柑皮乙醇提取物结晶、VC和PG分别是贡水白柚柚皮乙醇提取物结晶部分清除能力的17.407、1.586、0.514倍。在邻二氮菲体系中贡水白柚柚皮乙醇提取物对自由基的清除作用均弱于椪柑皮、PG和VC,其中椪柑皮乙醇提取物上清液、PG和VC分别是贡水白柚柚皮乙醇提取物上清液清除能力的18.416、4.820、1.716倍;而椪柑皮乙醇提取物结晶、PG和VC是贡水白柚柚皮乙醇提取物结晶清除能力的6.589、3.609、1.285倍。椪柑皮乙醇提取物上清液、PG和VC对超氧阴离子自由基的清除作用分别是贡水白柚柚皮乙醇提取物上清液的13.941、1.059、2.442倍;椪柑皮乙醇提取物结晶、PG和VC是贡水白柚柚皮乙醇提取物结晶的5.908、1.471、3.392倍。贡水白柚柚皮乙醇提取物具有一定的还原力,但弱于椪柑皮乙醇提取物、PG和VC。
In this study,the in vitro antioxidant effect of the 80% ethanol extract from Gongshui white pomelo peel was assessed and compared with those of the 80% ethanol extract from ponkan peel,VC,propyl gallate（PG）.The results were showed that the scavenging abilities of the 80% ethanol extract（supernatant） from ponkan peel,VC and PG against hydroxyl free radicals trapped with salicylic acid were 5.515 and 1.320 times higher and 0.428 lower than that of the 80% ethanol extract（supernatant） from Gongshui white pomelo peel,while the freeze dried crystal of 80% ethanol extract from ponkan peel,VC and PG were 17.407 and 1.586 times higher and 0.514 times lower than that of the freeze dried crystal of 80% ethanol extract from Gongshui white pomelo peel,respectively.The scavenging abilities of the 80% ethanol extract from ponkan peel,PG and VC against hydroxyl free radicals generated in Fe2＋/H2O2 reaction system were 18.416,4.820 and 1.716 times higher than that of the 80% ethanol extract from Gongshui white pomelo peel,and the hydroxyl free radical scavenging abilities of the freeze dried crystal of 80% ethanol extract from ponkan peel,PG and VC were 6.589,3.609 and 1.285 times higher than that of the freeze dried crystal of 80% ethanol extract from Gongshui white pomelo peel,respectively.In superoxide anion free radical scavenging ability,the 80% ethanol extract from ponkan peel,PG and VC were 13.941,1.059 and 2.442 higher than the 80% ethanol extract from Gongshui white pomelo peel and the freeze dried crystal of 80% ethanol extract from ponkan peel,PG and VC were 5.908,1.471 and 3.392 times higher than the freeze dried crystal of 80% ethanol extract from Gongshui white pomelo peel.The 80% ethanol extract from Gongshui white pomelo peel had the potential to reduce ferric ions,but its reducing power was weaker than those of 80% ethanol extract from ponkan peel,VC and PG.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第15期143-148,共6页
Food Science
基金
湖北省生物资源开发与利用重点实验室开放课题(2007016)
关键词
贡水白柚
椪柑
抗氧化作用
羟自由基
超氧阴离子自由基
还原力
Gongshui white pomelo
ponkan
antioxidation
hydroxy radical
superoxide anion radical
reducing power
作者简介
王威（1987-），男，本科生，研究方向为生物资源的开发与利用。E—mail：wangwei0730@163．com。
通信作者：程超（1976-），女，副教授，博士研究生，研究方向为生物资源的开发与利用。E-mail：chengchaow@126．com
