摘要
为研究吸附脱苦对堆溪蜜柚果汁中类胡萝卜素含量的影响，采用反相高效液相色谱法测定XAD-16、XAD-7HP、XAD-4这3种树脂吸附脱苦前、后瑁溪蜜柚果汁中类胡萝卜素成分的变化。试验结果表明：瑁溪蜜柚果汁中含有叶黄素、玉米黄质β-隐黄质、番茄红素邙一胡萝卜素5种类胡萝卜素；用高效液相色谱法分析琅溪蜜柚果汁中类胡萝卜素的加标回收率为95％～99％．相对标准偏差为1．3％-4．2％。经XAD-16树脂脱苦后，柚汁中的番茄红素、β-隐黄质、β-胡萝卜素、叶黄素、玉米黄质的损失率分别为85．2％，75．0％，70．8％，50．0％，48．7％；经XAD-7HP树脂脱苦后柚汁中以上5种类胡萝卜素损失率分别为78．6％，100％，76．9％，72：2％，65．1％；经XAD-4树脂脱苦后损失率分剐为75．1％，100％。71．7％，66．7％，61．7％。树脂脱苦可导致瑁溪蜜柚汁中类胡萝卜素含量显著降低。
The effect of adsorption debittering on earotenoids of Guanxi pomelo juice were studied. A HPLC method has been developed to determinate concentration changes in earotenoids of the pomelo juice after the Amberlite XAD-16, XAD-7HP, XAD-4 resin absorption. The result showed that the pomelo juice contained five carotenoids, namely lutein, zeaxanthin, lycopene, β-cryptoxanthin and β-carotene. The HPLC method had recoveries to these carotenoids in a range within 95% to 99%, and the relative standard deviations were 1.3%-4.2%. The XAD-16 resin treatment caused 85.2%, 75.0%, 70.8%, 50.0% and 48.7% decrease in the contents of lyeopene, β-cryptoxanthin, β-carotene, lutein, zeaxanthin in pomelo juice were; the XAD-7HP resin treatment absorbed 78.6%, 100%, 76.9%, 72.2% and 65.1% of lycopene, β-cryptoxanthin, β-carotene, lutein, zeaxanthin; and XAD-4 resin aborption decreased the lyeopene, β-cryptoxanthin, β-carotene, lutein and zeaxanthin by 78.6%, 100%, 76.9%, 72.2% and 65.1%, respectively. These results indicate that the absorption debittering dramatically reduce the content of carotenoids in the pomelo juice.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2014年第1期172-177,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31271914)
福建省基金项目(2011J01225)
厦门市杰出青年人才基金项目(3502Z20126008)
关键词
琯溪蜜柚
果汁
吸附脱苦
类胡萝卜素
高效液相色谱法
Guanxi pomelo
juice
adsorption debittering
carotenoids
high performance liquid chromatograph
作者简介
黄高凌，女，1966年出生，副教授
通讯作者：蔡慧农
