摘要
介绍了利用高产能源作物甜高粱生产燃料乙醇的先进固态发酵(ASSF)技术,从甜高粱茎秆保存、菌种、反应器,到固体发酵过程的数学模拟和工程放大进行了系统研究。筛选出高效产乙醇的菌种CGMCC1949,固体发酵时间低于30 h,乙醇收率高于92%;优选出贮存甜高粱茎秆的有效方法,通过抑菌处理,厌氧贮存200 d糖分损失小于5%;对固态发酵过程进行了数学模拟,设计并优化了固体发酵设备,成功进行了工程放大试验,并且基于ASPEN软件对该技术进行了技术经济评价,结果表明ASSF法生产甜高粱乙醇在技术、工程和经济上均具有充分的可行性和明显优势。
A robust strain of the species Saccharomyces cerevisiae CGMCC1949 was screened and identified, and advanced solid state fermentation （ASSF） technology for fuel ethanol production from sweet sorghum stalks was thus developed. The fermentation time was shortened to less than 30 h, and ethanol yield was 92% of its theoretical maximum. And in the meantime, the cost-effective storage was established for sweet sorghum stalks, with less than 5% sugar loss after 200 days of storage, making the plant operation could extend up to 200 days without feedstock shortage. With the fermentation kinetics and heat-mass transfer models, modeling of the ASSF process was investigated, and the rotating drum bioreactor was designed. Furthermore, the ASSF technology was successfully applied in the pilot plant in which the rotating drum bioreactor was scaled up to 127 m3, and ethanol yield of 91% was achieved. At the end, techno-economic analysis （TEA） conducted by ASPEN indicated that ethanol production from sweet sorghum stalks by the ASSF is economically competitive.
出处
《生物工程学报》
CAS
CSCD
北大核心
2010年第7期966-973,共8页
Chinese Journal of Biotechnology
基金
国家重大国际合作项目(No.S2010GR0393)
农业部948项目(No.2008-G2)资助~~
关键词
甜高粱
先进固态发酵
燃料乙醇
sweet sorghum stalks, advanced solid state fermentation （ASSF）, ethanol production
作者简介
Corresponding author： Shizhong Li. Tel： ＋86-10-62772123; Fax： ＋86-10-80194050; E-mail： Szli@tsinghua.edu.cn
