摘要
以自制壳聚糖为载体，戊二醛为交联剂将胰蛋白酶固定化。5％戊二醛在30℃下处理载体8h，加10ml酶液（0．3mg／ml，pH7．0）固定12h以上，活力回收率达67％－75％。固定化酶的表观米氏常数（酪蛋白）k'm=22．22mg／ml，而游离酶k'm=4.17mg／ml；固定化酶最适温度为80℃，比游离酶提高了30℃；固定化最适pH值为7．5，而游离酶为8．0；固定化酶的贮存稳定性很好，二个多月重复使用，酶活力未见明显下降。
Trypsin was irmobilized on chitosan and crosslinked with glutaraldehyde. The optimum conditions for immobilization were as follows: Chitosan was treated with 5% solution of qlutaraldehyde at 30℃ for 8h, then 10ml solution of trypsin (0.3mg/ml pH7. 0 ) was inunobilized on the carrier. Enzyme activity recovery was 67 %～75%. Apparent Michealis constant of ianobilized trypsln k'm=66.67mg/ml, and that of nature trypsin k'm=4.17mg/ml. The optimum temperature of immoilized trypsin was 80t℃, i. e 30℃ higher than natural trypsin 30℃. The optimum pH's for irmobilized trypsin and natural trypsin were 7.5 and 8.0 respectively. The stability of immobilized trysin was high after storage.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1999年第1期29-32,共4页
Food Science
关键词
壳聚糖
固定化
胰蛋白酶
Chitosan, Immobilizetion, Trypase
