摘要
以壳聚糖微球为载体,戊二醛为交联剂,固定β-半乳糖苷酶对β-半乳糖苷酶的固定化条件及固定化酶的各种性质进行研究,确定酶固定的最适条件为:用pH6.5的P-E-M缓冲液浸泡10h,25℃壳聚糖微球与0.5%戊二醛交联12h以上,4℃下酶与壳聚塘微球固定12h以上,酶活力回收率可达67%。固定化酶的最适温度为40℃左右,最适pH7.0。通过双倒数法求回归方程,求得酶动力学参数K_m值为0.613mmol/ml。固定化酶稳定性好,可以重复使用。将该固定化酶应用于乳糖分解实验和作为柱层析介质连续分解乳糖,分批反应6批次,乳糖水解率保持在90%以上,连续水解20d,乳糖水解率仍然可保持在75%以上。
β-Galactosidase was immobilized on chitosan microspheres with glutaraldehyde by cross-linking reaction,the immobilezation conditions and characterization of the immobilized enzyme were studide.The optimal conditions for immobi- lization were as follow:in pH 6.5 P-E-M solution for 10 h,chitosan micrnsphere was treated with 0.5% glutaraldehde at 25℃for 12h,the solution ofβ-galactosidase was immobilized on the carrier at 4℃for 12 h,enzyme activity recovery was 67%.Optimal temperature and pH for the immobilized...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第1期222-227,共6页
Food Science
关键词
壳聚糖微球
固定化
Β-半乳糖苷酶
操作稳定性
chitcsan
microspheres
immobilization
β-galactosidase
operational stability
