摘要
目的:建立毛细管气相色谱法测定药用辅料倍他环糊精中的残留溶剂。方法:采用顶空气相色谱法测定,色谱柱为DB-1石英毛细管柱(50 m×0.53 mm,5μm),载气为氮气。进样口温度为200℃,FID检测器,检测器温度为250℃,柱温为90℃,顶空进样,平衡温度为70℃,平衡时间为20 min,分流比为1:1;20%二甲基亚砜溶液为溶剂;二氯乙烯为内标物。结果:被测物得到很好分离,峰面积与浓度呈良好线性关系,精密度和回收率良好。结论:该方法可用于倍他环糊精中残留溶剂的检测。
Objective： To establish a method of head-space capillary gas chromatography（CGC） for the determination of residual solvents in betacyelodextrin. Method： The residual solvents in betacyclodextrin were separated by DB-1 capillary column （50 m × 0. 53 mm,5 μm）with an FID detector. The carried gas was nitrogen. The injector temperature and the detector temperature was controlled at 200 ℃ and 250 ℃, respectively. The column temperature was 90 ℃, The sample was dissolved in 20% dimethyl sulfoxide （DMSO）, and acetylenedichoride was used as the internal standard. The containers of head-space injector were in equilibrium at 70 ℃ for 20 min, the splitting-ratio was 1： 1. Result： The measured solvent were separated completely. The calibration curve of each solvent showed a good linear relationship with good precision and accuracy. Conclusion： The established method is accurate and reliable. It can be used for determination of residual solvents in betaeyelodextrin.
出处
《中国药师》
CAS
2010年第6期830-831,共2页
China Pharmacist
关键词
倍他环糊精
残留溶剂
毛细管气相色谱法
Betacyclodextrin
Residual solvent
Capillary gas chromatography
作者简介
通讯作者：周志敏Tel：13877173556 E—mail：NNZZM@Tom．com
