摘要
应用ISSR分析技术对19个省市的97株古银杏雄株进行遗传多样性分析,并对它们的亲缘关系进行分析鉴定。结果表明:银杏雄株具有丰富的基因多样性和遗传多样性,选用的13个引物共扩增出114条DNA条带,其中多态性DNA条带83条,占72.8%;有效等位基因数为1.8117,基因多样度为0.4374,Shannon信息指数为0.6253;利用UPGMA法对扩增结果进行聚类分析,把97株古银杏雄株分为2大类,一类表现出较强的地理相关性,另一大类结果表明遗传距离与地理位置远近不完全相关。
The genetic diversity of 97 ancient male ginkgo trees from 19 provinces was estimated by using ISSR markers to analyze the genetic relation of these individual trees.The results show that male ginkgo trees have high genetic diversity.Thirteen ISSR（inter-simple sequence repeat） primers produced a total of 114 bands,of which 83 were polymorphic.The percentage of polymorphic bands was 72.8% ,the effective number of alleles 1.811 7,Nei＇s genetic distance was 0.437 4 and Shannon index 0.625 3.UPGMA（unweighted pair-group method using arithmetic average） cluster analysis showed that the 97 individuals were divided into two groups.Group one was closely correlated with geographical locations, while for the other group,the genetic distance among the sampled male gingko trees was uncorrelated with geographical distance.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2010年第2期39-45,共7页
Journal of Beijing Forestry University
基金
"十一五"国家科技支撑计划项目(2006BAD18B0301)
关键词
银杏
雄株
ISSR
遗传多样性
Ginkgo biloba
male tree
ISSR
genetic diversity
作者简介
王国霞，博士。主要研究方向：经济林栽培。电话：025—85427994Email：wgxia191919@sina．com．cn地址：210037南京龙蟠路南京林业大学森林资源与环境学院。
责任作者：曹福亮，教授，博士生导师。主要研究方向：经济林栽培。电话：025-85420799Email：sameao@njfu．edu．cn地址：210037南京龙蟠路南京林业大学森林资源与环境学院。
