摘要
从牛蒡(Arctium lappa L.)的根、茎、叶、总苞、种子中分离纯化了57株内生菌。以牛蒡子粉为底物,采用高效液相色谱法定量测定转化培养液中牛蒡子苷和牛蒡子苷元的含量,筛选得到6株转化牛蒡子苷的内生菌。其中L2的转化率最高,可达76.9%。利用LC-MS方法对其转化产物牛蒡子苷元进行了验证。L2经过形态观察、生理生化特征分析及16S rDNA序列分析,鉴定为氧化微杆菌(Microbacterium oxydans)。本试验结果为探索牛蒡内生菌转化牛蒡子苷成主要药效成分牛蒡子苷元技术提供了科学依据。
To investigate the endophytic microorganisms to transform arctiin from Arctium lappa L. , endophytes were firstly isolated from surface-sterilized roots, stems, leaves, involucrum and seeds of Arctium lappa L. 6 of the 57 endophytes obtained from A. lappa showed a promising potential to hydrolyse arctiin. Among them, strain L2 showed the highest transformation ratio （76. 9%）. Bacterium L2 was identified as Microbacterium ozydans L2 by a polyphasic approach including 16 s rDNA sequencing and physiological and biochemical as well as morphological analysis. A HPLC-UV based method was used for the determination of aretiin and aretigenin in fermentation broth and the transformation product was identified as arctigenin by LC-MS. Our results suggested that it＇s an efficient way to find our microorganisms that can transform arctiin into arctigenin in vitro from the endophytes of Arctiurn lappa L. To our best knowledge, this is the first time to transform arctiin into its active form, actigenin, directly using seeds powder as substrate.
出处
《药物生物技术》
CAS
CSCD
2010年第2期111-116,共6页
Pharmaceutical Biotechnology
基金
国家十一五科技支撑计划项目(2006BAI09B20-6)
关键词
牛蒡子苷
牛蒡子苷元
微生物转化
内生菌
鉴定
氧化微杆菌
Arctiin, Arctigenin, Biotransformation, Endophytes, Identification Microbacterium oxyclans
作者简介
作者简介：张淼，女，1984年生，辽宁省沈阳市人，辽宁中医药大学和中国科学院沈阳应用生态研究所联合培养在读硕士研究生，主要从事药用植物微生物学研究。
通讯作者：胡江春，Email：hujc@iae．ac．cn；
窦德强；E—mail：doudeqiang2003@yahoo．com.cn。
