摘要
在单因素实验和多因数-二水平实验的基础上,采用Box-Benhnken实验设计,选取水溶性淀粉、酵母浸膏和培养时间为自变量,以荧光物质的总荧光强度作为因变量,通过响应面法优化了罗汉果内生真菌SG-59产荧光物质的培养条件。结果表明,此菌株产荧光物质的最佳培养条件为:水溶性淀粉36.5 g/L,酵母浸膏7.0 g/L,500 mL培养瓶装液量为170 mL,28℃静置培养27 d。这将为大规模发酵和进一步研究这些荧光物质打下基础。
Based on the single factor experiments and multi-factors-two-level experiments, the water- solubility starch, yeast extract, and cultivated time were selected as independent variables and the total fluorescence intensity was as the dependent variable using Box-Benhnken experimental design Finally the culture medium of a strain endophytic fungus SG-59 from Siraitia grosvenori producing fluorescent substance was optimized by response surface methodology. Results showed that the best culture conditions were as follow ： water-solubility starch 36.5 g/L, yeast extracts 7. 0 g/L, 500 mL bottle culture filled with 170 mL, static culture 27 days under the temperature of 28 ℃. This will lay foundations for the further study of those fluorescent after large-scale fermentation.
出处
《广西大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第2期241-244,共4页
Journal of Guangxi University（Natural Science Edition）
基金
广西自然科学基金资助项目(桂科自0728257)
广西大学博士科研启动项目
广西研究生教育创新计划项目(105930903027)
关键词
罗汉果内生真菌
荧光物质
培养基
优化
响应面法
Siraitia grosvenori endophytic fungus
fluorescent substance
culture media
optimization
Response Surface Methodology
作者简介
通讯联系人：陈海燕（1976-），男，湖南江华人，博士，广西大学副教授，硕士生导师；E—mail：czyzsu@gxu．edu．cn。
