摘要
对实验室保藏的一株高产Monacolin K红曲霉菌株及其野生株进行了初步的PKS基因结构分析。扩增得到红曲霉野生株W菌及其诱变株M菌的目的基因序列,并将测序产物和cDNA序列进行比对,确定该段基因序列不含内含子,且诱变株M菌的突变位置不在此段基因上。分别用ProfileScan、SWISS-MODEL预测了该段蛋白的结构域以及三维结构。
The PKS Gene structure of a lab-preserved Monasc us strain with high yield of Monacolin K and its wild strain was analyzed. Then the gene sequence of Monascus wild strain W and its mutant strain M were amplified by polymerase chain reaction （PCR）. And the sequencing products were compared with cDNA sequence. The results confirmed that such gene segment had no intron and the mutation position of mutant strain M did not locate on such gene segment. Finally, the domain and 3D structure of the protein in such segment was predicted by ProfileScan and SWISS-MODEL respectivley.
出处
《酿酒科技》
2010年第4期17-20,共4页
Liquor-Making Science & Technology
基金
863国家基金资助项目(024-D90661)
广东省自然科学基金资助项目(07006553)
作者简介
丘振宇（1981-），男，硕士研究生，从事发酵工程、生物化工方面的研究，发表论文10余篇。
