摘要
目的建立异丙基肾上腺素诱导SD大鼠充血性心力衰竭模型,观察血清醛固酮的变化。方法用ISO(170 mg/kg)对SD大鼠皮下注射2次,建立大鼠充血性心力衰竭模型,在造模后18周进行血流动力学检测左室舒张末压力、左室内压峰值、左心室压力上升/下降最大速率及心率,取血检测醛固酮,并取心肌观察病理形态学特征。结果与正常对照组相比,模型组大鼠心率及左室舒张末压力明显增加(P<0.01),左室内压峰值、左心室压力上升/下降最大速率明显下降(P<0.01),左心室重量指数明显增加(P<0.01),醛固酮显著升高(P<0.01)。光学显微镜结果显示,异丙基肾上腺素组大鼠心肌细胞肥大,部分心肌细胞有嗜酸变性和/或坏死,心肌间质结缔组织增生。结论应用大剂量异丙基肾上腺素诱导大鼠充血性心力衰竭模型,该作用可能与血浆ALD升高有关。
Objective To establish the animal model of isoproterenol （ISO）- induced congestive heart failure （CHF） in rats and study the changes of serum aldosterone. Methods Male Sprague- Dawley rats were randomly allocated to the ISO- induced chronic heart failure group （ISO group, n=50） treated with two subcutaneous injections of ISO and the healthy control group （CON group, n= 10） treated with two subcutaneous injections of normal saline. At the 18th week, the left ventricular end diastolic pressure, left ventricular end diastolic pressure, maximal rate of LV pressure rise/decline and heart rate were assessed. Aldosterone （ALD） in plasma was detected. 18 weeks later, the mass ratio of heart ventricle and left ventricle to body was measured. Results Compared with that in CON group, the LVEF, FS significantly decreased and the LVEDD, LVESD in ISO group enlarged notably （P〈0.01）. The mass ratio of left ventricle to body and the concentration of ALD of ISO group increased distinctly （P〈0. 01）, compared with that of CON group. Conclusion Activation of plasma aldosterone is increased in high-dose isoproterenol- induction rat model of CHF.
出处
《西部医学》
2010年第4期595-598,共4页
Medical Journal of West China
基金
南京军区南京总医院科研基金资助项目(批准号:Q2008042)
作者简介
通讯作者：蔡辉，Emall：gh．yz．js．chn@163．com
