摘要
[目的]研究鹊肾树(Streblus asperLour.)叶石油醚萃取物及其组分的体外抗肿瘤活性。[方法]采用MTT法检测鹊肾树叶石油醚萃取物以及经硅胶柱层析分离各组分的体外抗肿瘤活性。[结果]80μg/ml鹊肾树叶石油醚萃取物对宫颈癌细胞Hela、人胃癌细胞SGC-7901、口腔上皮癌细胞KB作用48 h的抑制百分率分别达到58.6%、53.5%和75.5%。进一步分离纯化鹊肾树叶的石油醚萃取物,所得的FrⅡ组分(80μg/ml)对Hela、SGC-7901和卵巢癌细胞Ca-cell的48 h抑制率分别达到69.7%、64.6%和60.2%,FrⅢ组分对Hela、SGC-7901和Ca-cell的48 h抑制率分别达到73.1%、66.1%和52.8%。[结论]鹊肾树叶石油醚萃取物具有一定的抗肿瘤作用,经进一步分离所得的FrⅡ、FrⅢ组分对体外肿瘤细胞增殖呈明显的抑制作用。
[Objective] The aim was to investigate anticancer effect in vitro of petroleum ether extract from leaves of Streblus asper Lour.and Its Components.[Method]MTT method was adopted to detect petroleum ether extract and anticancer effect in vitro of each component was separated by silica gel column chromatography.[Result]The inhibitive percentage of 80 μg/ml petroleum ether extract on Hela,SGC-7901 and KB cells were 58.6%,53.5%and 75.5%after 48 hours respectively.After further separation and purification of petroleum ether extract,the inhibitive percentage of fraction FrⅡ（80 μg/ml） on Hela,SGC-7901 and Ca-cell cells were 69.7%,64.6% and 60.2%after 48 hours respectively.And the inhibitive percentage of fraction FrⅢ（80 μg/ml） on Hela,SGC-7901 and Ca-cell cells were 73.1%,66.1% and 52.8%after 48 hours respectively.[Conclusion] Petroleum ether extract from leaves of Streblus asper Lour.has certain anticancer effect.And inhibitory effect of the fraction FrⅡ and FrⅢ seperated from petroleum ether extract on tumor cell proliferation is also obvious.
出处
《安徽农业科学》
CAS
北大核心
2010年第5期2340-2341,共2页
Journal of Anhui Agricultural Sciences
关键词
鹊肾树
抗肿瘤
MTT法
Streblus asper Lour.
Anticancer
MTT method
作者简介
梁成钦（1977-），男，广西平南人，硕士，助理研究员，从事天然药物化学研究。
通讯作者，教授，硕士生导师，E—mail．．xq5895801@163．com。
