摘要
通过蒸馏–沉淀法制备单分散的聚苯乙烯(PS)微球,并用两亲性聚合物聚乙二醇(PEG)对PS进行修饰.以牛血清蛋白(BSA)为模型,研究PS-PEG微球对BSA的吸附与解吸特性.结果表明,聚合物微球对BSA的吸附受pH、微球上PEG含量以及NaCl溶液质量浓度的影响,作用力为疏水吸附.在无盐水体系下解吸,解吸率最高为96.2%,表明微球在蛋白质分离应用中可重复使用.
Monodisperse polystyrene microspheres（PS）were prepared by distillation-precipitation polymerization and modified by amphipathic polymers polyethylene glycol （PEG）. Bovine serum albumin （BSA） was used as model proteins to investigate the adsorption and desorption properties on PS-PEG microspheres. As a result,BSA adsorption on polymer mi- crospheres was driven by hydrophobic interaction and effected by the value ofpH,the capacities of PEG on the microspheres and inorganic salt concentration. The maximum desorption ratios of BSA on PS-PEG was 96.2% at none salt aqueous. So the microspheres can be reused when they are used to separate proteins.
出处
《天津科技大学学报》
CAS
2010年第1期28-31,共4页
Journal of Tianjin University of Science & Technology
基金
天津科技大学引进人才科研启动基金资助项目(20060419)
关键词
聚合物微球
吸附
疏水作用
蛋白质
polymer microsphere
adsorption
hydrophobic interaction
protein
作者简介
程绍玲（1970-），女，吉林人，副教授，博士，yhcsl@tust．edul.cn．
