摘要
为了探讨肺炎克雷伯氏菌对喹诺酮类药物的耐药机制，从临床送检标本中分离出８株耐环丙沙星的肺炎克雷伯氏菌，对其外膜蛋白进行了ＳＤＳ－ＰＡＧＥ分析。结果发现除１株菌外，其余所有耐环丙沙星的肺炎克雷伯氏菌４２ｋＤ外膜蛋白带消失，另有１株菌的２５ｋＤ外膜蛋白带也同时消失。这一结果表明，肺炎克雷伯氏菌的４２ｋＤ外膜蛋白与其对环丙沙星的通透性有关。
To study the quinolone resistant mechanism in Klebsiella pneumoniae , we isolated 8 strains of Klebsiella pneumoniae resistant to ciprofloxacin from clinical specimens and analysed their outer membrane protein profile by SDS PAGE. Results demonstrated that the 42kD band disapeared from outer membrane proteins of all resistant strains except one, the 25kD protein band of one strain also disapeared. Our findings indicated that the 42kD outer membrane protein of Klebsiella pneumoniae is related to its permeation for ciprofloxacin and the disapearance of this protein is one of the important reasons for Klebsiella pneumoniae resistant to ciprofloxacin.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1998年第5期380-382,共3页
Chinese Journal of Antibiotics
关键词
肺炎克雷伯氏菌
环丙沙星
外膜蛋白
耐药
Klebsiella pneumoniae
Ciprofloxacin
Outer membrane protein
Resistance
