摘要
猪血红蛋白水解液中多肽、血红素等营养成分浓度较低,有必要对猪血红蛋白水解液进行浓缩。用0.2μm的陶瓷微滤膜和截留相对分子质量3.5×103的超滤膜对猪血红蛋白水解液进行微滤澄清和超滤浓缩,考察膜滤前后水解液中粗多肽、血红素等成分的含量变化及膜的各项性能表征。结果表明,0.2μm陶瓷膜对猪血红蛋白水解液有明显的澄清效果,粗多肽得率为76.150%,血红素得率为80.154%,膜再生效果好,膜通量恢复率达到97.42%。超滤对粗多肽和血红素的浓缩倍数分别达到3.5倍和3倍。因此,微/超滤技术适用于浓缩猪血红蛋白水解液,能获得富含多肽和血红素的浓缩物。
It was necessary to condensate porcine hemoglobin hydrolysates due to lower concentration of nutritional peptides and heme in hydrolysates. The ceramic microfiltration membrane with 0.2 μm pores and ultrafiltration membrane with 3.5 × 10^3 cut-off were used for clarification and condensation of hemoglobin hydrolysates, respectively. Contents and characterizations of peptides and heme in hydrolysates after microfiltration and ultrafiltration were investigated. Results showed that the ceramic microfiltration membrane with 0.2 μm pores revealed a significant clarification effect on porcine hemoglobin hydrolysates and provided 76.150% recovery rate for peptides and 80.154% recovery rate for heme. In addition, an excellent regeneration for membrane was observed and the recovered membrane flux was achieved up to 97.42%. Compared to the original hydrolysates, the concentration of peptides and heme in treated hydrolysates by microfiltration and ultrafiltration was increased by 3.5 and 3 folds, respectively. Therefore, microfiltration and ultrafiltration technology could be used for the condensation of porcine hemoglobin hydrolysates to gain peptides-and heme-enriched hydrolysates.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第24期26-29,共4页
Food Science
基金
国家自然科学基金项目(30270976)
关键词
微/超滤技术
浓缩
血红蛋白
血红素
多肽
microfiltration/ultrafiltration technology
condensation
hemoglobin
heme
peptides
作者简介
杨万根（1974-），男，讲师，博士，研究方向为食品生物技术与畜禽水产深加工。E—mail：yangwangen08@163．com
通讯作者：马美湖（1957-），男，教授，博士，研究万向为食品生物技术。E—mail：mameihuhn@yahoo．com．cn
