摘要
目的:建立孕14～20周甲胎蛋白(AFP)中位数,并使用唐氏综合征阳性病例进行验证分析。方法:使用AFP时间分辨免疫荧光法试剂检测21 278份正常单胎孕妇血清和21例唐氏综合征(DS)阳性孕妇血清。根据末次月经日期确定样本孕周,采用直接法计算AFP各孕周的中位数。以此为基础,计算阳性病例中倍数(MoM),并进行统计学分析。结果:AFP中位数水平与美国PE公司提供的白种人孕妇数据相比差异有显著性(P=0.000);分析DS病例,其母血AFP MoM值均值与正常孕妇相比差异有显著性(P=0.000)。结论:本研究建立的中位数可以区分阳性和阴性病例,能有效应用于风险评估。
Objective To establish the medians of alpha-fetoprotein （AFP） in second trimester （14 - 20 weeks） and verify the values using Down＇s syndrome （DS）positive cases. Methods 21 278 maternal serum samples of normal singleton pregnancies and 21 cases of DS were detected using time-resolved fluoroimmunoassay （TRFIA）. Based on last menstrual period （LMP）, the gestational age was recorded . The median values of AFP during 14 - 20 gestational weeks were calculated by direct counting. The AFP concentrations of positive cases were converted to multiples of the median （MoM） at the relevant gestational age. Results The median values of AFP were significant higher in Chinese ethnicity group established in the present study than those of white groups provided by PerkinElmer（P = 0.000）. Significant differences of the means of MoM were found between DS cases and the normal ones （P = 0.000）. Conclusion The AFP median values established in the present study can.be effectively used to discriminate high-risk pregnancies from nomal ones.
出处
《实用医学杂志》
CAS
北大核心
2009年第24期4144-4146,共3页
The Journal of Practical Medicine
基金
广州市重大科技攻关项目(编号:2005Z1-E4031)
作者简介
通信作者：李明E-mail：mingli2006_2006@126．com
