摘要
利用红叶石楠叶片和茎段作为外植体进行愈伤组织试验,研究了TDZ和AgNO3及其他植物生长调节剂对红叶石楠愈伤组织诱导的影响效果。结果表明:TDZ和AgNO3对红叶石楠愈伤组织诱导具有一定的促进作用,但需NAA配合,方能形成愈伤组织。本试验中,培养基1/2MS+AgNO32.5 mg/l+TDZ0.5 mg/l+NAA2.0 mg/l对叶片有较好诱导效果,1/2MS+AgNO32.5 mg/l+TDZ0.5 mg/l+NAA1.0 mg/l对茎段有较好诱导效果。此外,1.5 mg/l的TDZ与0.5 mg/l的2,4-D配比有较好诱导效果。光培养条件下,叶片的诱导率高于茎段;在暗培养下,茎段的诱导率高于叶片。
The leaf and stem explants of Pbotinia x fraseri were used to induce the callus with TDZ, AgNO3 and other plant regulator. The resuits showed that TDZ and AgNO3 have promote effect on calluses induction of Photinia x fraseri explants,but they need NAA to combinne. The best medium in this study for callus induction is 1/2MS ＋ AgNO32.5 mg/1 ＋ TDZO. 5 mg/1 ＋ NAA2.0 mg/l by leaf, 1/2MS ＋ Ag- NO32.5 mg/1 ＋ TDZ0. 5 mg/1 ＋ NAA1. 0 mg/l by stem. Besides, 1.5 mg/l TDZ and 5 mg/l 2,4-D could induce callus. The more callus were induced of the leaf explants than of the stem under light,on the contrary,the callus of the stem is more than of the leaf under dark.
出处
《世界科技研究与发展》
CSCD
2009年第6期986-988,985,共4页
World Sci-Tech R&D
基金
四川省教育厅(2006C-048)
绵阳师范学院科技处(MA2005008)
作者简介
李明银（LIMingyin），博士，研究方向：植物资源与遗传育种。
