摘要
目的建立改良组织消化和原子吸收光谱测定组织中铂浓度的方法。方法应用岛津原子吸收光谱系统,条件:AA-6300型原子吸收分光光度计、GFA-Ex7i石墨炉、ASC-6100自动进样器、铂元素空心阴极灯,含载顺铂磁性纳米药物的组织烘干后经硝酸和双氧水水浴消化后直接采用原子吸收光谱仪在265.9nm处测定。结果各不同组织顺铂线性范围均是54～283.5μg.L-1,相关系数r均大于0.999,日内变异系数均小于5%,日间变异系数均小于10%。结论采用改良原子吸收光谱法可高效、准确的检测湿化消化法处理的组织样本中铂元素的含量,该改良方法适用于顺铂药物动力学的研究。
Aim To establish a modified method of tissue digestion and cis-platin concentration measurement in tissue by atomic absorption spectrometry.Methods Using shimadzu atomic absorption spectrometry system and under the conditions of AA-6300 atomic absorption spectrophotometer,GFA-Ex7i graphite furnace,ASC-6100 automatic sampler and platinum hollow cathode discharge lamp,the cisplatin concentration of the tissues containing loading cisplatin magnetic nanomedicine which were digested with nitric acid and hydrogen peroxide in water bath,was measured by atomic absorption spectrophotometer at 265.9 nm.Results The concentrations of cis-platin in different tissues were in the linear range of 54~283.5 μg·L-1;all the correlation coefficient were larger than 0.999 and all the inter-day and intro-day variation coefficient were smaller than 5%.Conclusion The modified method of tissue digestion and atomic absorption spectrometry applied is of high precision and efficiency, suitable for the pharmacokinetic research on cis-platin.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第11期1513-1516,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30672297)
广东省科技计划资助项目(No2006B35802002)
广东省自然科学基金重点项目(No36645)
广州市科技计划资助项目(No2004Z3-D2-91)
关键词
顺铂
原子吸收光谱
磁性纳米药物
含量测定
改良
小鼠
组织
cis-platin
atomic absorption spectrometry
magnetic nanomedicine
concentration measurement
modification
mouse
tissue
作者简介
王蕾（1981-），女，硕士，医师，研究方向：鼻咽癌早期诊断，E—mail：leileiwl@126．com；
谢民强（1956-），男，博士，教授，研究方向：以鼻咽癌为主的头颈肿瘤早期诊断和治疗，Tel：020-61643389，E-mail：xiemqgz@pub．guangzhou．gd．cn
